Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   SMSK321_RS08405 Genome accession   NZ_AEDT01000026
Coordinates   7872..8696 (+) Length   274 a.a.
NCBI ID   WP_000359013.1    Uniprot ID   -
Organism   Streptococcus mitis SK321     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2872..13696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMSK321_RS08370 (SMSK321_1096) asnA 2962..3954 (+) 993 WP_000748007.1 aspartate--ammonia ligase -
  SMSK321_RS08375 (SMSK321_1097) rsmD 4019..4558 (+) 540 WP_004235374.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  SMSK321_RS08380 (SMSK321_1098) coaD 4548..5036 (+) 489 WP_001280735.1 pantetheine-phosphate adenylyltransferase -
  SMSK321_RS08385 (SMSK321_1099) sepM 5020..6057 (+) 1038 WP_000727281.1 SepM family pheromone-processing serine protease Regulator
  SMSK321_RS08390 (SMSK321_1100) - 6115..6345 (+) 231 WP_000250395.1 DUF1146 family protein -
  SMSK321_RS08395 (SMSK321_1101) murA 6369..7652 (+) 1284 WP_000411905.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  SMSK321_RS08400 (SMSK321_1102) - 7645..7833 (+) 189 WP_004241151.1 DNA-directed RNA polymerase subunit beta -
  SMSK321_RS08405 (SMSK321_1103) endA 7872..8696 (+) 825 WP_000359013.1 DNA-entry nuclease EndA Machinery gene
  SMSK321_RS08410 (SMSK321_1104) - 8964..10295 (+) 1332 WP_000390025.1 hemolysin family protein -
  SMSK321_RS08415 (SMSK321_1106) amiA 10718..12697 (+) 1980 WP_000749707.1 peptide ABC transporter substrate-binding protein Regulator

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29896.55 Da        Isoelectric Point: 9.9431

>NTDB_id=64505 SMSK321_RS08405 WP_000359013.1 7872..8696(+) (endA) [Streptococcus mitis SK321]
MDKKTRQTLIGLLVLLLLSAGSYYIKQMQSTANSPKTKVSQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNREETGNGSTSWTPPGWHQVKNLKGTYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEDSTGQNYYESMVRKTLDKNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=64505 SMSK321_RS08405 WP_000359013.1 7872..8696(+) (endA) [Streptococcus mitis SK321]
ATGGATAAAAAGACAAGACAGACACTGATCGGACTGCTAGTGTTATTGCTTTTATCTGCGGGAAGCTATTATATCAAGCA
GATGCAGTCGACAGCTAATAGTCCTAAAACAAAGGTTAGTCAGAAAAAACAAGCGTCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAAGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTT
AATGGCAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGATAATAAAACAAAGACAGTAGGCAAGGA
AACAGTGCCAACCGTAGCAAATGCCCTCTTGTCTAAGGCTACTCGCCAGTACAAGAATCGTGAAGAAACTGGGAATGGTT
CGACTTCTTGGACTCCTCCAGGCTGGCATCAGGTTAAGAATCTAAAGGGTACTTATACCCATGCGGTTGATAGAGGTCAC
TTGTTAGGCTATGCCTTAATCGGTGGCTTGGATGGTTTTGATGCCTCGACAAGCAATCCTAAAAACATTGCTGTTCAAAC
AGCCTGGGCAAATCAGGCGCAAGCCGAGGATTCGACTGGTCAAAACTACTATGAAAGTATGGTACGTAAGACCTTGGATA
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCGAATGAGGATTTAGTTCCTTCAGCTTCACAGATTGAA
GCCAAGTCTTCAGACGGAGAATTGGAATTTAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTGACTGTTACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

95.255

100

0.953
  endA Streptococcus pneumoniae D39

95.255

100

0.953
  endA Streptococcus pneumoniae R6

95.255

100

0.953
  endA Streptococcus pneumoniae TIGR4

95.255

100

0.953

Multiple sequence alignment