Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   L0Z22_RS02365 Genome accession   NZ_CP090737
Coordinates   502488..503993 (-) Length   501 a.a.
NCBI ID   WP_048025671.1    Uniprot ID   A0A8E1SCJ7
Organism   Burkholderia cepacia strain 2011Ycf427V     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 497488..508993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0Z22_RS02340 (L0Z22_02340) - 498514..499149 (-) 636 WP_021162603.1 LON peptidase substrate-binding domain-containing protein -
  L0Z22_RS02345 (L0Z22_02345) rapZ 499177..500085 (-) 909 WP_021162602.1 RNase adapter RapZ -
  L0Z22_RS02350 (L0Z22_02350) hprK 500161..501129 (-) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  L0Z22_RS02355 (L0Z22_02355) - 501261..501800 (-) 540 WP_034195946.1 PTS sugar transporter subunit IIA -
  L0Z22_RS02360 (L0Z22_02360) raiA 501970..502329 (-) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  L0Z22_RS02365 (L0Z22_02365) rpoN/rpoN1 502488..503993 (-) 1506 WP_048025671.1 RNA polymerase factor sigma-54 Machinery gene
  L0Z22_RS02370 (L0Z22_02370) lptB 504165..504941 (-) 777 WP_021162599.1 LPS export ABC transporter ATP-binding protein -
  L0Z22_RS02375 (L0Z22_02375) lptA 504938..505603 (-) 666 WP_021162598.1 lipopolysaccharide transport periplasmic protein LptA -
  L0Z22_RS02380 (L0Z22_02380) lptC 505624..506238 (-) 615 WP_021162597.1 LPS export ABC transporter periplasmic protein LptC -
  L0Z22_RS02385 (L0Z22_02385) - 506242..506778 (-) 537 WP_021162596.1 HAD family hydrolase -
  L0Z22_RS02390 (L0Z22_02390) kdsD 506778..507761 (-) 984 WP_249453085.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54797.94 Da        Isoelectric Point: 4.8767

>NTDB_id=644578 L0Z22_RS02365 WP_048025671.1 502488..503993(-) (rpoN/rpoN1) [Burkholderia cepacia strain 2011Ycf427V]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNSGSSSSERAERDEPAGADEYDGYASGDGNDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=644578 L0Z22_RS02365 WP_048025671.1 502488..503993(-) (rpoN/rpoN1) [Burkholderia cepacia strain 2011Ycf427V]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGTGTCGCAGCGGACGGATCGTTGATCGCGCAGACGCCTCCCGCCCAGAACACCGAGCCCGCTGCG
ACAAACAGTGGCAGTTCGTCGAGCGAGCGCGCCGAGCGCGACGAACCGGCCGGCGCGGACGAATACGACGGTTACGCGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAACCTCGACGAATTCGGCCGCGCGAGCGGCGCGTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAATTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGCGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCACTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACACGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGCTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCGATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGGTTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.379

100

0.691