Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   L0Y81_RS13075 Genome accession   NZ_CP090718
Coordinates   533086..534591 (-) Length   501 a.a.
NCBI ID   WP_006398255.1    Uniprot ID   -
Organism   Burkholderia multivorans strain 2014Y70532416II     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 528086..539591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0Y81_RS13050 (L0Y81_13050) - 529113..529749 (-) 637 Protein_507 LON peptidase substrate-binding domain-containing protein -
  L0Y81_RS13055 (L0Y81_13055) rapZ 529777..530685 (-) 909 WP_006409809.1 RNase adapter RapZ -
  L0Y81_RS13060 (L0Y81_13060) hprK 530764..531732 (-) 969 WP_006398252.1 HPr(Ser) kinase/phosphatase -
  L0Y81_RS13065 (L0Y81_13065) - 531861..532373 (-) 513 WP_035488861.1 PTS sugar transporter subunit IIA -
  L0Y81_RS13070 (L0Y81_13070) raiA 532568..532927 (-) 360 WP_006398254.1 ribosome-associated translation inhibitor RaiA -
  L0Y81_RS13075 (L0Y81_13075) rpoN/rpoN1 533086..534591 (-) 1506 WP_006398255.1 RNA polymerase factor sigma-54 Machinery gene
  L0Y81_RS13080 (L0Y81_13080) lptB 534744..535520 (-) 777 WP_006398256.1 LPS export ABC transporter ATP-binding protein -
  L0Y81_RS13085 (L0Y81_13085) lptA 535517..536182 (-) 666 WP_006398257.1 lipopolysaccharide transport periplasmic protein LptA -
  L0Y81_RS13090 (L0Y81_13090) lptC 536203..536817 (-) 615 WP_006398258.1 LPS export ABC transporter periplasmic protein LptC -
  L0Y81_RS13095 (L0Y81_13095) - 536821..537357 (-) 537 WP_006398259.1 HAD family hydrolase -
  L0Y81_RS13100 (L0Y81_13100) kdsD 537357..538340 (-) 984 WP_006398260.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54746.94 Da        Isoelectric Point: 4.8740

>NTDB_id=644489 L0Y81_RS13075 WP_006398255.1 533086..534591(-) (rpoN/rpoN1) [Burkholderia multivorans strain 2014Y70532416II]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPVSNGEPAI
ANGNGQSSDRAERDEPAGADEYDGYAAGDASDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWQAELNPEVVP
KLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=644489 L0Y81_RS13075 WP_006398255.1 533086..534591(-) (rpoN/rpoN1) [Burkholderia multivorans strain 2014Y70532416II]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTGGCGATGGCGGTCGCCCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTTGCGGCGGACGGCTCGCTGATCGCGCAGACGCCGCCCGTGTCGAACGGCGAGCCGGCCATC
GCGAACGGCAACGGCCAGTCGAGCGATCGGGCGGAGCGCGACGAACCGGCCGGCGCCGACGAATACGACGGCTATGCGGC
CGGCGACGCGAGCGACGGCCCGCAGTGGAACCTCGACGAATTCGGCCGCGCGAGCGGCGCCTCCGACGACGACGATCTGC
CGCCGCTGCAGGTACAGGAAGCGGCGACGTCGCTGCGCGATCATCTGTCCGCGCAGCTGCGCGTCACGCAGGCGAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTGACCGCGTCGCTCGACGAGGTGCT
CGCCGATCTGCCGCCCGAACTCGAAGTCGACTGCGATGAACTGAACGCGGCGCTCGCACTGCTGCACAGCTTCGATCCGG
CCGGCGTCGGCGCGCGTTCGGCATCGGAATGCCTGAAGCTGCAGCTGCTGCGGCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAACATCTGGAACTGCTCGCCGCGCGCGATTTCACGCGGTTGCGCAAGCATCTGAAGGCCAC
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCCTACGGCAAGGCCGAAG
CCGACTACGTGGTGCCCGACATCATCGTGAAGAAAGTCGGCCAGGGCTGGCAGGCCGAACTCAATCCCGAGGTCGTGCCG
AAGCTGCGCATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGCTCGCTGAAGCAGCA
GCTGCAGGAAGCGCGCTGGCTCATCAAGAACATCCAGCAGCGGTTCGACACGATCCTGCGCGTCGCGCAGGCGATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACGGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCGCACGTCTCGACCGACACCGGTGGCGCCGCATCGTCGACCGCCATCCGTGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCCCAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGCTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAGGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.775

100

0.695