Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   L0Z16_RS27025 Genome accession   NZ_CP090680
Coordinates   2923711..2925216 (+) Length   501 a.a.
NCBI ID   WP_105774153.1    Uniprot ID   -
Organism   Burkholderia multivorans strain 2019Y71421880II     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2918711..2930216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0Z16_RS27000 (L0Z16_27000) kdsD 2919962..2920945 (+) 984 WP_249485583.1 arabinose 5-phosphate isomerase KdsD -
  L0Z16_RS27005 (L0Z16_27005) - 2920945..2921481 (+) 537 WP_006406621.1 HAD family hydrolase -
  L0Z16_RS27010 (L0Z16_27010) lptC 2921485..2922099 (+) 615 WP_006398258.1 LPS export ABC transporter periplasmic protein LptC -
  L0Z16_RS27015 (L0Z16_27015) lptA 2922120..2922785 (+) 666 WP_006398257.1 lipopolysaccharide transport periplasmic protein LptA -
  L0Z16_RS27020 (L0Z16_27020) lptB 2922782..2923558 (+) 777 WP_006398256.1 LPS export ABC transporter ATP-binding protein -
  L0Z16_RS27025 (L0Z16_27025) rpoN/rpoN1 2923711..2925216 (+) 1506 WP_105774153.1 RNA polymerase factor sigma-54 Machinery gene
  L0Z16_RS27030 (L0Z16_27030) raiA 2925375..2925734 (+) 360 WP_006398254.1 ribosome-associated translation inhibitor RaiA -
  L0Z16_RS27035 (L0Z16_27035) - 2925929..2926441 (+) 513 WP_035488861.1 PTS sugar transporter subunit IIA -
  L0Z16_RS27040 (L0Z16_27040) hprK 2926570..2927538 (+) 969 WP_249485585.1 HPr(Ser) kinase/phosphatase -
  L0Z16_RS27045 (L0Z16_27045) rapZ 2927617..2928525 (+) 909 WP_006409809.1 RNase adapter RapZ -
  L0Z16_RS27050 (L0Z16_27050) - 2928553..2929188 (+) 636 WP_053296824.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54756.98 Da        Isoelectric Point: 4.8740

>NTDB_id=644336 L0Z16_RS27025 WP_105774153.1 2923711..2925216(+) (rpoN/rpoN1) [Burkholderia multivorans strain 2019Y71421880II]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPVPNGEPAI
ANGNGQSSDRAERDEPAGADEYDGYAAGDASDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWQAELNPEVVP
KLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=644336 L0Z16_RS27025 WP_105774153.1 2923711..2925216(+) (rpoN/rpoN1) [Burkholderia multivorans strain 2019Y71421880II]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTGGCGATGGCGGTCGCCCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGTCCGCTGCGCGTTGCGGCGGACGGCTCGCTGATCGCGCAGACGCCGCCCGTGCCGAACGGCGAGCCGGCCATC
GCGAACGGCAACGGCCAGTCGAGCGATCGTGCGGAGCGCGACGAACCGGCCGGCGCCGACGAATACGACGGCTACGCGGC
CGGCGACGCGAGCGACGGCCCGCAGTGGAACCTCGACGAATTCGGCCGCGCGAGCGGCGCCTCCGACGACGACGATCTGC
CGCCGCTGCAGGTACAGGAAGCGGCGACGTCGCTGCGCGATCATCTGTCCGCGCAGCTGCGCGTCACGCAGGCAAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTGACCGCATCGCTCGACGAGGTGCT
CGCCGATCTGCCGCCCGAACTCGAAGTCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGCGTCGGCGCGCGTTCGGCATCGGAATGCCTGAAGCTGCAGCTGCTGCGGCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAACATCTGGAACTGCTCGCCGCGCGCGATTTCACGCGGTTGCGCAAGCATCTGAAGGCCAC
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCCTACGGCAAGGCCGAAG
CCGACTACGTGGTGCCCGACATCATCGTGAAGAAAGTCGGCCAGGGCTGGCAGGCCGAACTCAATCCCGAGGTCGTGCCG
AAGCTGCGCATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGCTCGCTGAAGCAGCA
GCTGCAGGAAGCGCGCTGGCTCATCAAGAACATCCAGCAGCGGTTCGACACGATCCTGCGCGTCGCGCAGGCGATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACGGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCGCACGTCTCGACCGACACCGGTGGCGCCGCATCGTCGACCGCCATCCGTGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCCCAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGCTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAGGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.515

100

0.691