Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LXE93_RS04460 Genome accession   NZ_CP090632
Coordinates   977921..979426 (-) Length   501 a.a.
NCBI ID   WP_046544390.1    Uniprot ID   -
Organism   Burkholderia contaminans strain FFH2055     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 972921..984426
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LXE93_RS04435 (LXE93_04425) - 973950..974585 (-) 636 WP_039346030.1 LON peptidase substrate-binding domain-containing protein -
  LXE93_RS04440 (LXE93_04430) rapZ 974613..975521 (-) 909 WP_046544388.1 RNase adapter RapZ -
  LXE93_RS04445 (LXE93_04435) hprK 975597..976565 (-) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  LXE93_RS04450 (LXE93_04440) - 976695..977234 (-) 540 WP_046544389.1 PTS sugar transporter subunit IIA -
  LXE93_RS04455 (LXE93_04445) raiA 977403..977762 (-) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  LXE93_RS04460 (LXE93_04450) rpoN/rpoN1 977921..979426 (-) 1506 WP_046544390.1 RNA polymerase factor sigma-54 Machinery gene
  LXE93_RS04465 (LXE93_04455) lptB 979586..980362 (-) 777 WP_046544391.1 LPS export ABC transporter ATP-binding protein -
  LXE93_RS04470 (LXE93_04460) lptA 980359..981024 (-) 666 WP_039346006.1 lipopolysaccharide transport periplasmic protein LptA -
  LXE93_RS04475 (LXE93_04465) lptC 981045..981659 (-) 615 WP_046544392.1 LPS export ABC transporter periplasmic protein LptC -
  LXE93_RS04480 (LXE93_04470) - 981663..982199 (-) 537 WP_046544393.1 HAD family hydrolase -
  LXE93_RS04485 (LXE93_04475) kdsD 982199..983182 (-) 984 WP_046544394.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54695.80 Da        Isoelectric Point: 4.8740

>NTDB_id=644118 LXE93_RS04460 WP_046544390.1 977921..979426(-) (rpoN/rpoN1) [Burkholderia contaminans strain FFH2055]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
ANGSSSSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=644118 LXE93_RS04460 WP_046544390.1 977921..979426(-) (rpoN/rpoN1) [Burkholderia contaminans strain FFH2055]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGTGTCGCTGCGGACGGATCGCTGATCGCGCAGACGCCTCCCGCGCAGAACACCGAGCCCGCTGCG
GCAAACGGTAGCAGCTCGTCGAGCGATCGTGCCGAGCGCGACGAGCCGGCCGGCGCGGACGAATACGACGGTTACGGGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAACCTCGACGAATTCGGCCGCTCGAGCGGCGCATCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGATGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGGGTCGGTGCCCGTTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGTTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTTTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCACAGGCGATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCTTCGTCGACAGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCTAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGATTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.577

100

0.693