Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HRR25_RS11770 Genome accession   NZ_AP021896
Coordinates   2423652..2424329 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain 2018-02-2CC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2418652..2429329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HRR25_RS11735 (VEGS05_23130) gspG 2419132..2419569 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  HRR25_RS11740 (VEGS05_23140) gspH 2419577..2420086 (+) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  HRR25_RS11745 (VEGS05_23150) gspI 2420083..2420460 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  HRR25_RS11750 (VEGS05_23160) gspJ 2420453..2421040 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  HRR25_RS11755 (VEGS05_23170) gspK 2421033..2422016 (+) 984 WP_172410671.1 type II secretion system minor pseudopilin GspK -
  HRR25_RS11760 (VEGS05_23180) gspL 2422031..2423194 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  HRR25_RS11765 (VEGS05_23190) gspM 2423191..2423652 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  HRR25_RS11770 (VEGS05_23200) pilD 2423652..2424329 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  HRR25_RS11775 (VEGS05_23210) bfr 2424358..2424834 (-) 477 WP_000675504.1 bacterioferritin -
  HRR25_RS11780 (VEGS05_23220) bfd 2424906..2425100 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  HRR25_RS11785 (VEGS05_23230) chiA 2425269..2427962 (-) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=64403 HRR25_RS11770 WP_000178161.1 2423652..2424329(+) (pilD) [Escherichia coli strain 2018-02-2CC]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=64403 HRR25_RS11770 WP_000178161.1 2423652..2424329(+) (pilD) [Escherichia coli strain 2018-02-2CC]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment