Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   L0B74_RS01855 Genome accession   NZ_CP090603
Coordinates   369522..370898 (+) Length   458 a.a.
NCBI ID   WP_249905800.1    Uniprot ID   -
Organism   Lactobacillus amylovorus strain S1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 364522..375898
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0B74_RS01835 - 365542..366912 (-) 1371 WP_249905438.1 IS4 family transposase -
  L0B74_RS01840 pepC 367124..368473 (-) 1350 WP_013437164.1 aminopeptidase C -
  L0B74_RS01845 - 368535..368840 (-) 306 WP_013641492.1 hypothetical protein -
  L0B74_RS01850 - 368971..369522 (+) 552 WP_046433038.1 dUTP diphosphatase -
  L0B74_RS01855 radA 369522..370898 (+) 1377 WP_249905800.1 DNA repair protein RadA Machinery gene
  L0B74_RS01860 gltX 370975..372474 (+) 1500 WP_013641493.1 glutamate--tRNA ligase -
  L0B74_RS01865 cysS 372571..374004 (+) 1434 WP_013437169.1 cysteine--tRNA ligase -
  L0B74_RS01870 - 373997..374440 (+) 444 WP_013641495.1 ribonuclease III domain-containing protein -
  L0B74_RS01875 rlmB 374427..375179 (+) 753 WP_013437171.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  L0B74_RS01880 - 375318..375863 (+) 546 WP_013641496.1 sigma-70 family RNA polymerase sigma factor -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50382.93 Da        Isoelectric Point: 6.4078

>NTDB_id=643887 L0B74_RS01855 WP_249905800.1 369522..370898(+) (radA) [Lactobacillus amylovorus strain S1]
MARVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIKKTGINEPVKLDKIKAEKEERIPTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDLSEKYKVLYVSGEESANQIKLRADRLGVGQSNMLLYPETDMHDI
REQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITIFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHSYRILHSVKNRFGAANEIGMFEMENEGLKEVTNPSSIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGLDYNRAALLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAIVMAVSSSYTDKEISPTDCFVGEVGL
TGEVRRVDKIDARVKEAAKVGFKRIFVPRHNMYAGLQDYGIEVIPVSSIPQALKLVFD

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=643887 L0B74_RS01855 WP_249905800.1 369522..370898(+) (radA) [Lactobacillus amylovorus strain S1]
ATGGCAAGAGTAAAAACACAATATAAATGTCGCTCCTGTGGCTATATTTCTGCGAGTTACTTGGGTAGATGCCCTAATTG
TGGTGCTTGGAATCAGTTTGAAAAAGAGACTGAAGAAGTACAGAAGCGTTCAACTAAGGCAACTGCTAGTCGTTTAATTA
AGAAAACTGGAATTAATGAGCCAGTCAAATTAGATAAAATAAAAGCTGAAAAAGAAGAACGAATTCCTACCAAATCTGAA
GAATTAAATCGTGTTTTAGGTGGCGGTATCGTGCCAGGATCGCTTGTCTTAATTGGCGGGGATCCCGGAATTGGTAAATC
TACATTGATGCTGCAGATCATGAGCGATTTATCTGAAAAATATAAAGTACTATATGTTTCAGGGGAAGAATCAGCTAATC
AGATCAAGTTAAGAGCCGATCGACTTGGCGTAGGGCAAAGCAACATGCTGTTATATCCCGAAACTGACATGCATGATATT
CGTGAACAAATTAATGATGTTAAGCCTGATTTTGTGGTTATCGATTCAATTCAAACTATGAATGAACCAAGTCTTGATTC
GATGACAGGCTCTGCTTCTCAAGTACGTGAAGTAACTAGTGAGTTAATGAAAATTGCCAAGATGGATGCAATTACTATTT
TTGTAATTGGACATGTAACTAAAGAAGGTGCAATCGCTGGTCCAAAGATCTTAGAGCACATGGTTGACACAGTTCTTTAT
TTTGAAGGTGATGAACATCATTCTTACAGAATTCTGCATTCAGTTAAAAACCGTTTTGGAGCTGCTAACGAAATAGGGAT
GTTTGAAATGGAAAATGAAGGATTAAAAGAAGTAACTAATCCTTCTTCAATTTTCCTTGATCAAAGATTACCGAATTCCA
CCGGCTCTGCTGTCGTTGTTTCGCTTGAAGGAACCCGTCCACTTTTAGCTGAAATCCAAGCTTTGGTGACCCCAACGGCA
TTTGGCTATGCGAAGCGAACAACTTCAGGCCTTGATTACAATCGAGCAGCTCTTTTACTTGCCGTTCTTGAAAAGCGTGG
CAATTTAATGTTGCAAAATCAAGATGTTTATTTAACTGCAACTGGCGGGATTAAACTGAATGAACCGGCAATTGATTTAG
CTATTGTTATGGCTGTGTCATCTAGTTACACAGATAAAGAAATTTCACCAACGGACTGCTTTGTAGGTGAAGTTGGTTTA
ACCGGTGAAGTTCGCCGCGTAGACAAGATCGATGCTAGGGTAAAAGAAGCCGCTAAAGTTGGCTTTAAACGAATCTTTGT
ACCGCGGCACAACATGTATGCTGGTCTTCAGGATTATGGCATCGAAGTAATTCCAGTTTCCAGTATTCCACAAGCATTAA
AATTAGTTTTTGATTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

AAA domain (AAA_25)

(66-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

57.768

99.782

0.576
  radA Streptococcus mitis NCTC 12261

57.768

99.782

0.576
  radA Streptococcus pneumoniae Rx1

57.768

99.782

0.576
  radA Streptococcus pneumoniae D39

57.768

99.782

0.576
  radA Streptococcus pneumoniae R6

57.768

99.782

0.576
  radA Streptococcus pneumoniae TIGR4

57.768

99.782

0.576
  radA Bacillus subtilis subsp. subtilis str. 168

55.604

99.345

0.552