Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   OF379_RS19705 Genome accession   NZ_CP107284
Coordinates   4085018..4085458 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain SW4848     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4080018..4090458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OF379_RS19685 (OF379_19685) aroP 4081016..4082389 (+) 1374 WP_072146659.1 aromatic amino acid transporter AroP -
  OF379_RS19690 (OF379_19690) ampE 4082432..4083286 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  OF379_RS19695 (OF379_19695) ampD 4083283..4083834 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OF379_RS19700 (OF379_19700) nadC 4083922..4084815 (+) 894 WP_001135190.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OF379_RS19705 (OF379_19705) pilA 4085018..4085458 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  OF379_RS19710 (OF379_19710) pilB 4085468..4086853 (+) 1386 WP_001025166.1 type II secretion system protein GspE Machinery gene
  OF379_RS19715 (OF379_19715) hofC 4086843..4088045 (+) 1203 WP_000157245.1 protein transport protein HofC -
  OF379_RS19720 (OF379_19720) guaC 4088080..4089123 (-) 1044 WP_001217337.1 GMP reductase -
  OF379_RS19725 (OF379_19725) - 4089279..4089323 (-) 45 WP_120795372.1 protein YacM -
  OF379_RS19730 (OF379_19730) coaE 4089348..4089968 (+) 621 WP_001269517.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=643454 OF379_RS19705 WP_000360904.1 4085018..4085458(+) (pilA) [Escherichia coli strain SW4848]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=643454 OF379_RS19705 WP_000360904.1 4085018..4085458(+) (pilA) [Escherichia coli strain SW4848]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGTAATGGCATCCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGTTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363