Detailed information NTG0643354

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: OH217_RS14865
• Genome accession: NZ_CP107281
• Coordinates: 2986804..2987796 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081588.1
• Uniprot ID: Q540P0
• Organism: Escherichia coli strain JCL301
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 2981804..2992796

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OH217_RS14845 (OH217_14755)	otnK	2982580..2983746 (+)	1167	WP_001393459.1	3-oxo-tetronate kinase	-
OH217_RS14850 (OH217_14760)	ygbL	2983838..2984476 (+)	639	WP_001278994.1	aldolase	-
OH217_RS14855 (OH217_14765)	ygbM	2984481..2985257 (+)	777	WP_001136934.1	HPr family phosphocarrier protein	-
OH217_RS14860 (OH217_14770)	ygbN	2985346..2986710 (+)	1365	WP_000104441.1	GntP family transporter	-
OH217_RS14865 (OH217_14775)	rpoS	2986804..2987796 (-)	993	WP_000081588.1	RNA polymerase sigma factor RpoS	Regulator
OH217_RS14870 (OH217_14780)	nlpD	2987859..2988953 (-)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
OH217_RS14875 (OH217_14785)	pcm	2989138..2989764 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
OH217_RS14880 (OH217_14790)	surE	2989758..2990519 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
OH217_RS14885 (OH217_14795)	truD	2990500..2991549 (-)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
OH217_RS14890 (OH217_14800)	ispF	2991546..2992025 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
OH217_RS14895 (OH217_14805)	ispD	2992025..2992735 (-)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37971.86 Da
• Isoelectric Point: 4.5886

>NTDB_id=643354 OH217_RS14865 WP_000081588.1 2986804..2987796(-) (rpoS) [Escherichia coli strain JCL301]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEQEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=643354 OH217_RS14865 WP_000081588.1 2986804..2987796(-) (rpoS) [Escherichia coli strain JCL301]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAACAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

• Sigma-70 region 3 (Sigma70_r3) (174-248)

3D structure

• AlphaFold DB: Q540P0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715