Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   OH217_RS00540 Genome accession   NZ_CP107281
Coordinates   117109..117549 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain JCL301     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 112109..122549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OH217_RS00515 (OH217_00515) coaE 112599..113219 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  OH217_RS00520 (OH217_00520) - 113244..113288 (+) 45 WP_120795372.1 protein YacM -
  OH217_RS00525 (OH217_00525) guaC 113444..114487 (+) 1044 WP_001217338.1 GMP reductase -
  OH217_RS00530 (OH217_00530) hofC 114522..115724 (-) 1203 WP_000157266.1 protein transport protein HofC -
  OH217_RS00535 (OH217_00535) pilB 115714..117099 (-) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  OH217_RS00540 (OH217_00540) pilA 117109..117549 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  OH217_RS00545 (OH217_00545) nadC 117752..118645 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OH217_RS00550 (OH217_00550) ampD 118733..119284 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OH217_RS00555 (OH217_00555) ampE 119281..120153 (+) 873 WP_317566175.1 beta-lactamase regulator AmpE -
  OH217_RS00560 (OH217_00560) - 120126..120230 (+) 105 Protein_111 2-oxo acid dehydrogenase subunit E2 -
  OH217_RS00565 (OH217_00565) lpdA 120555..121979 (+) 1425 WP_000102485.1 dihydrolipoyl dehydrogenase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=643313 OH217_RS00540 WP_000360904.1 117109..117549(-) (pilA) [Escherichia coli strain JCL301]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=643313 OH217_RS00540 WP_000360904.1 117109..117549(-) (pilA) [Escherichia coli strain JCL301]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363