Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HRR26_RS02040 Genome accession   NZ_AP021895
Coordinates   450535..451212 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain 2018-11-3CC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 445535..456212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HRR26_RS02025 (VEGS11_04120) chiA 446878..449595 (+) 2718 WP_172410848.1 bifunctional chitinase/lysozyme -
  HRR26_RS02030 (VEGS11_04130) bfd 449764..449958 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  HRR26_RS02035 (VEGS11_04140) bfr 450030..450506 (+) 477 WP_000675504.1 bacterioferritin -
  HRR26_RS02040 (VEGS11_04150) pilD 450535..451212 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  HRR26_RS02045 (VEGS11_04160) gspM 451212..451673 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  HRR26_RS02050 (VEGS11_04170) gspL 451670..452833 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  HRR26_RS02055 (VEGS11_04180) gspK 452848..453831 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  HRR26_RS02060 (VEGS11_04190) gspJ 453824..454411 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  HRR26_RS02065 (VEGS11_04200) gspI 454404..454781 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  HRR26_RS02070 (VEGS11_04210) gspH 454778..455287 (-) 510 WP_172410849.1 type II secretion system minor pseudopilin GspH -
  HRR26_RS02075 (VEGS11_04220) gspG 455295..455732 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=64296 HRR26_RS02040 WP_000178161.1 450535..451212(-) (pilD) [Escherichia coli strain 2018-11-3CC]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=64296 HRR26_RS02040 WP_000178161.1 450535..451212(-) (pilD) [Escherichia coli strain 2018-11-3CC]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment