Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   IHC91_RS00980 Genome accession   NZ_CP090489
Coordinates   203444..205171 (+) Length   575 a.a.
NCBI ID   WP_394327968.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain AS-15-3942-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 198444..210171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHC91_RS00960 (IHC91_00960) pilM 200673..201671 (+) 999 WP_237029641.1 type IV pilus biogenesis protein PilM -
  IHC91_RS00965 (IHC91_00965) - 201668..202309 (+) 642 WP_237029642.1 PilN domain-containing protein -
  IHC91_RS00970 (IHC91_00970) - 202306..202896 (+) 591 WP_068969671.1 type 4a pilus biogenesis protein PilO -
  IHC91_RS00975 (IHC91_00975) - 202905..203420 (+) 516 WP_065172419.1 pilus assembly protein PilP -
  IHC91_RS00980 (IHC91_00980) pilQ 203444..205171 (+) 1728 WP_394327968.1 type IV pilus secretin PilQ Machinery gene
  IHC91_RS00985 (IHC91_00985) aroK 205501..206019 (+) 519 WP_036765246.1 shikimate kinase AroK -
  IHC91_RS00990 (IHC91_00990) aroB 206049..207128 (+) 1080 WP_044174096.1 3-dehydroquinate synthase -
  IHC91_RS00995 (IHC91_00995) - 207143..208651 (+) 1509 WP_237029643.1 SPOR domain-containing protein -
  IHC91_RS01000 (IHC91_01000) dam 208759..209601 (+) 843 WP_005302664.1 adenine-specific DNA-methyltransferase -
  IHC91_RS01005 (IHC91_01005) - 209585..209782 (-) 198 WP_036764707.1 DUF2970 domain-containing protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62754.23 Da        Isoelectric Point: 4.9029

>NTDB_id=642824 IHC91_RS00980 WP_394327968.1 203444..205171(+) (pilQ) [Photobacterium damselae subsp. damselae strain AS-15-3942-8]
MKRMLSVSLGWQRCWVVMLCCWLGLSQAAIAAGMNIVNNQITDLDFKRDNRGRGVLTVQLAKPQSLVDMRRSGKQLHIDI
FDTAIKDDMLYVLDVVDFATVVRSIETFRTEKGSKLILNMAGVFDYDYQLKGSRLEVVLNAQKKKTSSTAKAKQGKIDYK
GKPISINFQDVPVRNVLQLVAEYNDFNLVVSDTVQGNVTLRLDDVPWPQVLDIILQSKGLDKRVQGSVVLIAPKSELATN
ERQEMEAKRKEDELASLRSELIQINYAKAGDIADLLLGTKDGVSMLSQRGSLHVDERTNAIIIKDIPESIDSIEEIITAL
DIPVKQVQIEARIVTVDEGDIDELGVRWGVVNQNGSTTIGGSIEGNLASSGLLGDDATVDDFLNVNLGAITPGASSIAFQ
VAKLGDILLDLELSALQAESKAEIISSPRLVTTNKKAAYIEQGTEIPYLEAASSGAVSVDFKKAVLSLMVTPQITPDDKL
VLDLLVTQDKVGETVVTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIYQHQVNNSVRKVPLLGDIPGLGVLFRHKLQEMG
KRELLIFVTPKIITQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=642824 IHC91_RS00980 WP_394327968.1 203444..205171(+) (pilQ) [Photobacterium damselae subsp. damselae strain AS-15-3942-8]
ATGAAAAGGATGTTATCGGTCAGCTTGGGTTGGCAACGTTGTTGGGTAGTTATGTTGTGCTGCTGGCTTGGTCTCTCCCA
AGCTGCCATTGCTGCTGGCATGAATATTGTGAATAACCAAATCACCGACTTAGATTTTAAGCGTGATAATCGTGGACGAG
GCGTGCTCACTGTTCAGTTAGCCAAACCTCAATCTCTGGTTGATATGCGCCGTTCAGGGAAGCAATTACATATCGATATC
TTTGATACCGCGATTAAAGATGACATGTTGTATGTTCTCGATGTAGTCGACTTTGCAACGGTTGTTCGTTCGATTGAAAC
ATTTCGCACCGAGAAAGGCAGTAAGCTGATTCTCAATATGGCTGGTGTGTTTGATTATGATTACCAATTAAAAGGCTCCC
GTCTTGAAGTTGTTTTGAACGCCCAGAAAAAGAAAACCAGTAGTACTGCCAAGGCTAAGCAAGGCAAAATTGATTATAAA
GGTAAGCCTATTTCCATTAACTTCCAAGATGTTCCTGTGCGTAACGTACTGCAGTTGGTTGCTGAATATAATGATTTTAA
TCTCGTGGTTTCCGATACCGTGCAGGGTAATGTGACCTTACGTCTTGATGATGTGCCTTGGCCACAAGTGCTCGATATTA
TTTTGCAATCAAAAGGGTTGGATAAACGAGTTCAAGGTTCTGTGGTACTGATTGCGCCAAAATCTGAGTTGGCAACCAAT
GAGCGTCAAGAGATGGAAGCTAAACGTAAAGAAGATGAGTTAGCTTCGCTTCGTTCGGAGCTGATTCAAATTAATTACGC
TAAAGCAGGAGATATCGCAGACCTTTTACTCGGAACGAAAGATGGAGTCAGTATGCTATCTCAACGTGGATCTTTGCATG
TTGATGAGCGTACTAATGCCATTATTATCAAAGATATCCCTGAGAGTATTGATAGCATTGAAGAGATTATCACAGCATTA
GATATTCCCGTTAAACAGGTTCAGATAGAAGCTCGTATTGTTACTGTTGATGAGGGGGATATTGACGAGTTAGGGGTACG
TTGGGGTGTCGTTAATCAAAACGGTAGTACAACCATTGGTGGCTCAATTGAAGGTAACCTGGCCAGTTCGGGCTTATTGG
GTGATGACGCGACTGTTGATGATTTCTTAAATGTAAATTTAGGAGCCATTACTCCAGGGGCATCGAGTATTGCATTTCAG
GTAGCAAAACTGGGCGATATTTTGCTCGATCTTGAATTATCCGCTCTACAGGCAGAATCAAAAGCAGAGATTATTTCTAG
TCCTCGATTAGTGACTACCAATAAAAAAGCGGCCTACATCGAACAAGGTACTGAGATCCCTTATTTAGAAGCGGCGTCCA
GTGGTGCGGTATCGGTAGACTTTAAAAAGGCAGTATTGAGTTTGATGGTCACACCACAAATTACCCCAGATGATAAATTG
GTGCTCGATCTGCTGGTCACGCAAGATAAAGTAGGGGAAACAGTTGTGACCGGAACCGGAGAAGCGGTCGCTATTGATAC
TCAACGAATTGGCACGCAAGTTCTGGTCAATAACGGCGAGACTGTGGTCTTAGGTGGTATCTACCAGCATCAGGTGAATA
ATTCTGTCCGTAAAGTCCCTTTGCTGGGTGATATTCCAGGATTGGGCGTATTGTTCCGTCATAAGCTGCAAGAGATGGGC
AAGCGTGAACTGTTGATTTTCGTCACACCAAAAATTATCACCCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(260-326)

Bacterial type II and III secretion system protein (Secretin)

(415-573)

Secretin and TonB N terminus short domain (STN)

(186-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

61.121

99.304

0.607
  pilQ Vibrio cholerae strain A1552

61.121

99.304

0.607
  pilQ Vibrio campbellii strain DS40M4

60.573

97.043

0.588
  pilQ Legionella pneumophila strain ERS1305867

42.647

94.609

0.403
  pilQ Legionella pneumophila strain Lp02

42.647

94.609

0.403
  comQ Acinetobacter baylyi ADP1

40.212

98.609

0.397
  pilQ Pseudomonas aeruginosa PAK

40.787

97.217

0.397
  pilQ Acinetobacter baumannii D1279779

39.138

100

0.395
  pilQ Acinetobacter baumannii strain A118

39.138

100

0.395