Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LXM88_RS20080 Genome accession   NZ_CP090483
Coordinates   3244830..3246338 (+) Length   502 a.a.
NCBI ID   WP_262536511.1    Uniprot ID   -
Organism   Burkholderia sp. S-53     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3239830..3251338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LXM88_RS20055 (LXM88_20005) kdsD 3241073..3242056 (+) 984 WP_262536503.1 arabinose 5-phosphate isomerase KdsD -
  LXM88_RS20060 (LXM88_20010) - 3242056..3242592 (+) 537 WP_262536505.1 KdsC family phosphatase -
  LXM88_RS20065 (LXM88_20015) lptC 3242596..3243210 (+) 615 WP_262536507.1 LPS export ABC transporter periplasmic protein LptC -
  LXM88_RS20070 (LXM88_20020) lptA 3243231..3243896 (+) 666 WP_262536509.1 lipopolysaccharide transport periplasmic protein LptA -
  LXM88_RS20075 (LXM88_20025) lptB 3243893..3244669 (+) 777 WP_096471805.1 LPS export ABC transporter ATP-binding protein -
  LXM88_RS20080 (LXM88_20030) rpoN/rpoN1 3244830..3246338 (+) 1509 WP_262536511.1 RNA polymerase factor sigma-54 Machinery gene
  LXM88_RS20085 (LXM88_20035) hpf 3246496..3246855 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  LXM88_RS20090 (LXM88_20040) - 3247024..3247563 (+) 540 WP_262536579.1 PTS sugar transporter subunit IIA -
  LXM88_RS20095 (LXM88_20045) hprK 3247693..3248661 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  LXM88_RS20100 (LXM88_20050) rapZ 3248737..3249645 (+) 909 WP_262536593.1 RNase adapter RapZ -
  LXM88_RS20105 (LXM88_20055) - 3249673..3250308 (+) 636 WP_047899051.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54826.93 Da        Isoelectric Point: 4.8767

>NTDB_id=642750 LXM88_RS20080 WP_262536511.1 3244830..3246338(+) (rpoN/rpoN1) [Burkholderia sp. S-53]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIVSPLRVAADGSLIAQTPPGQNTEPAP
SNSSGSTSGDRAERDEPAGADEYEGYGSGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAG
PRDRALVMFLIESLDDDGYLTATLDEVLADLPSELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRT
LALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVV
PRLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=642750 LXM88_RS20080 WP_262536511.1 3244830..3246338(+) (rpoN/rpoN1) [Burkholderia sp. S-53]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAACAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGTAAGCCCGCTGCGCGTCGCCGCGGACGGATCGCTGATCGCGCAGACACCTCCCGGCCAGAACACCGAGCCGGCCCCG
TCAAACAGCAGCGGCAGCACGTCGGGCGATCGCGCGGAGCGCGACGAGCCGGCCGGCGCCGACGAGTACGAAGGTTATGG
GTCCGGCGACGGTAACGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCTCGAGCGGCGCGTCCGACGACGACGACC
TCCCGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGTGTCACGCAGGCCGGC
CCGCGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGT
GCTCGCCGACCTGCCGTCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACC
CGGCCGGGGTCGGTGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACGCGCACG
CTCGCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCACGCGACTTCACGCGGTTGCGCAAGCACCTGAAGGC
GAGCGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCG
AGGCCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGTCAGGGATGGCTCGCGGAACTCAATCCGGAGGTCGTG
CCGCGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTGAAGCA
GCAGCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTG
TCGAACGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCAGCCGTGTGACAACAGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTA
CTTCTTCGGATCACACGTTTCAACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGA
TAGGAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTACGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.119

100

0.685