Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   OGY64_RS20955 Genome accession   NZ_CP107234
Coordinates   4247849..4248289 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain REF1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4242849..4253289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OGY64_RS20930 (OGY64_20845) coaE 4243339..4243959 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  OGY64_RS20935 (OGY64_20850) - 4243984..4244028 (+) 45 WP_120795372.1 protein YacM -
  OGY64_RS20940 (OGY64_20855) guaC 4244184..4245227 (+) 1044 WP_001217320.1 GMP reductase -
  OGY64_RS20945 (OGY64_20860) hofC 4245262..4246464 (-) 1203 WP_000157289.1 protein transport protein HofC -
  OGY64_RS20950 (OGY64_20865) gspE 4246454..4247839 (-) 1386 Protein_4096 type II secretion system protein GspE -
  OGY64_RS20955 (OGY64_20870) pilA 4247849..4248289 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  OGY64_RS20960 (OGY64_20875) nadC 4248493..4249386 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OGY64_RS20965 (OGY64_20880) ampD 4249474..4250025 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OGY64_RS20970 (OGY64_20885) ampE 4250022..4250876 (+) 855 WP_000171980.1 beta-lactamase regulator AmpE -
  OGY64_RS20975 (OGY64_20890) aroP 4250919..4252289 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=642630 OGY64_RS20955 WP_000360914.1 4247849..4248289(-) (pilA) [Escherichia coli strain REF1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=642630 OGY64_RS20955 WP_000360914.1 4247849..4248289(-) (pilA) [Escherichia coli strain REF1]
ATGGACAAGCAACGCGGTTTTACACTTATTGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363