Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LZ683_RS00550 Genome accession   NZ_CP090445
Coordinates   123917..125302 (-) Length   461 a.a.
NCBI ID   WP_182285218.1    Uniprot ID   -
Organism   Comamonas testosteroni strain X13     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 118917..130302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZ683_RS00520 (LZ683_00510) - 118965..119729 (+) 765 WP_182285213.1 glycosyltransferase family 25 protein -
  LZ683_RS00525 (LZ683_00515) - 119719..120852 (-) 1134 WP_275053810.1 glycosyltransferase -
  LZ683_RS00530 (LZ683_00520) - 120915..121880 (+) 966 WP_275054864.1 capsular polysaccharide synthesis protein -
  LZ683_RS00535 (LZ683_00525) - 121925..122134 (-) 210 WP_003059801.1 zinc-finger domain-containing protein -
  LZ683_RS00540 (LZ683_00530) - 122261..123199 (-) 939 WP_182285216.1 branched-chain amino acid transaminase -
  LZ683_RS00545 (LZ683_00535) - 123281..123736 (-) 456 WP_182285217.1 glycerate kinase -
  LZ683_RS00550 (LZ683_00540) radA 123917..125302 (-) 1386 WP_182285218.1 DNA repair protein RadA Machinery gene
  LZ683_RS00560 (LZ683_00550) - 125725..127032 (-) 1308 WP_182285219.1 Glu/Leu/Phe/Val dehydrogenase -
  LZ683_RS00565 (LZ683_00555) - 127242..128165 (+) 924 WP_256495383.1 fumarylacetoacetate hydrolase family protein -
  LZ683_RS00570 (LZ683_00560) - 128320..129183 (-) 864 WP_182285221.1 PPK2 family polyphosphate kinase -
  LZ683_RS00575 (LZ683_00565) - 129278..130273 (-) 996 WP_182285222.1 tripartite tricarboxylate transporter substrate binding protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 48103.42 Da        Isoelectric Point: 7.6670

>NTDB_id=642487 LZ683_RS00550 WP_182285218.1 123917..125302(-) (radA) [Comamonas testosteroni strain X13]
MAKEKTTFTCNACGGTSPRWLGKCPGCGAWNTLVETVADSASGGKNRLSQPQGYAGLANAQPVTPLSAIEAQDVARTPSG
IEELDRVLGGGVVEGGVVLIGGDPGIGKSTLLLQAMDALHRIGLPTLYVTGEESGAQVALRSRRLGLDNSQVNVLAEIQL
EKILATVEATQPAVVVIDSIQTVYSDQLSSAPGSVAQVRECAAHLTRAAKTTGITIILVGHVTKDGALAGPRVLEHMVDT
VLYFEGDTHSNFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVTNPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQALVD
QGGPAARRLSVGLDRDRLAMLLAVLNRHAGVACADQDVFVNAVGGVRINEPAADLAVMLAITSSLRGKSLPKGFIAFGEV
GLAGEVRPAPRGQERLKEAAKLGFTIAVVPKANAPKKPITGLTIHAVERVDEAINIVRGIG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=642487 LZ683_RS00550 WP_182285218.1 123917..125302(-) (radA) [Comamonas testosteroni strain X13]
ATGGCCAAAGAGAAAACCACTTTCACCTGCAACGCCTGTGGCGGCACCAGCCCGCGCTGGCTGGGCAAATGCCCGGGCTG
CGGCGCCTGGAACACCCTGGTCGAGACCGTGGCCGACTCCGCCTCGGGCGGCAAGAACCGCCTGAGCCAGCCCCAGGGCT
ATGCAGGTCTGGCCAATGCCCAGCCCGTCACCCCGTTGTCGGCCATCGAGGCCCAGGATGTGGCGCGCACCCCCAGCGGC
ATCGAGGAGCTGGACCGCGTGCTGGGCGGCGGCGTGGTGGAAGGCGGCGTGGTGCTGATTGGCGGAGACCCTGGAATAGG
TAAGTCCACACTTCTTTTGCAGGCCATGGATGCATTGCATCGCATAGGCCTGCCCACGCTCTATGTAACGGGCGAGGAAA
GCGGCGCCCAGGTGGCGCTGCGCTCGCGCCGGCTCGGGCTGGACAACAGCCAGGTCAATGTGCTGGCCGAGATCCAGCTC
GAGAAAATCCTCGCCACCGTGGAGGCCACCCAGCCCGCCGTGGTGGTGATCGACTCGATCCAGACCGTGTATTCGGACCA
GCTCTCCTCGGCTCCGGGATCCGTGGCCCAGGTACGCGAATGCGCGGCCCATCTGACGCGCGCGGCCAAGACCACGGGCA
TCACCATCATTCTGGTGGGCCATGTGACCAAGGATGGCGCGCTGGCCGGCCCGCGCGTGCTCGAGCACATGGTGGACACG
GTGCTTTACTTCGAAGGCGACACGCACAGCAACTTCCGTCTGGTACGCGCCATCAAGAACCGCTTCGGTGCCGTCAACGA
GATCGGTGTCTTCGCGATGACGGAAAAAGGCCTCAAGGGCGTGACCAATCCCAGCGCAATCTTCCTGAGCCAGCACAGCG
AACCCGTGCCCGGCAGCTGCGTGCTGGTGACGCTGGAGGGCACGCGCCCCATGCTGGTGGAAATCCAGGCGCTGGTGGAC
CAGGGCGGCCCGGCCGCGCGCCGCCTCTCCGTCGGGCTGGACCGCGACCGTCTGGCCATGCTGCTGGCCGTGCTCAACCG
CCATGCGGGCGTGGCCTGCGCCGACCAGGATGTCTTCGTCAATGCCGTGGGCGGCGTGCGCATCAATGAGCCTGCAGCCG
ACCTGGCAGTGATGTTGGCAATCACTTCCAGTTTGCGCGGCAAGTCGCTGCCCAAGGGCTTTATCGCGTTTGGCGAGGTC
GGCCTGGCCGGCGAGGTGCGCCCCGCCCCTCGCGGTCAGGAGCGTCTCAAGGAAGCAGCAAAGCTGGGATTCACTATCGC
CGTGGTTCCCAAGGCCAATGCCCCAAAGAAGCCCATCACCGGCCTGACCATCCATGCCGTGGAGCGCGTTGATGAAGCCA
TCAACATCGTGCGCGGCATTGGTTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-226)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(364-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.02

99.566

0.488
  radA Streptococcus mitis SK321

48.77

96.963

0.473
  radA Streptococcus pneumoniae D39

48.661

97.18

0.473
  radA Streptococcus pneumoniae TIGR4

48.661

97.18

0.473
  radA Streptococcus pneumoniae R6

48.661

97.18

0.473
  radA Streptococcus pneumoniae Rx1

48.661

97.18

0.473
  radA Streptococcus mitis NCTC 12261

48.546

96.963

0.471