Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M497_RS01695 Genome accession   NZ_CP090409
Coordinates   361903..363282 (+) Length   459 a.a.
NCBI ID   WP_003656608.1    Uniprot ID   -
Organism   Lactobacillus gasseri 2016     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 356903..368282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M497_RS01665 (M497_01665) - 357559..357816 (+) 258 WP_003655837.1 hypothetical protein -
  M497_RS01670 (M497_01670) - 357818..358003 (+) 186 WP_003647798.1 hypothetical protein -
  M497_RS01675 (M497_01675) - 358022..358699 (+) 678 WP_025012200.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  M497_RS01680 (M497_01680) - 358809..359744 (+) 936 WP_003647795.1 AAA family ATPase -
  M497_RS01685 (M497_01685) pepC 359809..361158 (-) 1350 WP_003655851.1 aminopeptidase C -
  M497_RS01690 (M497_01690) - 361352..361903 (+) 552 WP_003647794.1 dUTP diphosphatase -
  M497_RS01695 (M497_01695) radA 361903..363282 (+) 1380 WP_003656608.1 DNA repair protein RadA Machinery gene
  M497_RS01700 (M497_01700) gltX 363364..364863 (+) 1500 WP_003647793.1 glutamate--tRNA ligase -
  M497_RS01705 (M497_01705) cysS 364957..366369 (+) 1413 WP_025012201.1 cysteine--tRNA ligase -
  M497_RS01710 (M497_01710) - 366362..366805 (+) 444 WP_003647791.1 Mini-ribonuclease 3 -
  M497_RS01715 (M497_01715) rlmB 366792..367547 (+) 756 WP_003647790.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50067.70 Da        Isoelectric Point: 7.9629

>NTDB_id=642145 M497_RS01695 WP_003656608.1 361903..363282(+) (radA) [Lactobacillus gasseri 2016]
MAKVKTRYKCRNCGYISASYLGRCPNCGAWNQFEEETQEVKKVSTKATASRLMTKIGNNDPVKLNEVKAEKEKRIVTPFE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQITGALAKEHSVLYVSGEESASQIKMRADRLGVSNSGILLYPETNMQNI
REQIDEIKPDFLVIDSIQTMNEPSLDSMVGSASQVREVTSELMKIAKNDQITTFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDGHHSYRILRSVKNRFGAANEIGMFEMKNEGLTEVNNPSAIFLDERLPNSTGSAVVVSLEGTRPLLADIQALVTPTA
FGYAKRTTSGLDFNRVALLLAVLEKRGNLMLQNQDAFLTATGGIKLNEPAIDLAICMAVASSYKNKEISATDCFVGEVGL
TGEIRRVNQIEARVKEAAKVGFKRIFIPKHNLTTELKNNSAIEVIGVASLPQALKLVFN

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=642145 M497_RS01695 WP_003656608.1 361903..363282(+) (radA) [Lactobacillus gasseri 2016]
ATGGCAAAAGTTAAAACCCGTTACAAGTGTCGCAATTGTGGTTATATTTCAGCTTCTTATTTAGGTAGATGCCCTAACTG
CGGTGCTTGGAATCAATTTGAAGAAGAAACTCAAGAAGTAAAAAAAGTATCAACTAAGGCAACTGCCAGCCGTTTAATGA
CTAAAATTGGAAATAATGATCCAGTAAAATTAAATGAAGTAAAAGCTGAAAAAGAAAAAAGAATTGTAACGCCGTTTGAA
GAATTAAATCGAGTTCTAGGGGGCGGAATAGTTCCAGGTTCTTTAGTTTTAATTGGTGGAGATCCTGGAATTGGCAAATC
AACTTTGATGCTCCAAATTACCGGCGCTTTAGCTAAAGAGCATAGCGTTTTATATGTTTCAGGGGAAGAGTCGGCTAGTC
AGATAAAAATGCGAGCTGACCGTCTAGGTGTAAGCAACAGTGGAATTTTGCTTTATCCTGAAACCAATATGCAGAATATT
CGTGAACAGATTGATGAGATTAAGCCGGATTTTTTAGTAATCGATTCTATTCAAACAATGAATGAGCCATCTCTTGATTC
GATGGTAGGATCTGCTTCTCAAGTTCGAGAAGTTACGAGTGAGTTAATGAAAATTGCTAAGAATGATCAGATTACTACTT
TTGTTATTGGACATGTGACTAAAGAAGGTGCTATTGCTGGTCCTAAGATTATGGAACATATGGTTGACACTGTTCTTTAC
TTTGAGGGGGATGGCCACCATTCATATCGAATTTTAAGATCTGTTAAAAATCGTTTTGGGGCAGCTAATGAAATTGGCAT
GTTTGAAATGAAAAATGAAGGACTAACAGAAGTGAATAATCCTTCAGCAATTTTCTTAGACGAACGCTTACCCAATTCTA
CAGGGTCAGCAGTTGTAGTTTCGCTTGAAGGAACAAGGCCACTTTTAGCAGATATTCAGGCCTTGGTAACTCCAACTGCC
TTTGGATATGCTAAAAGAACTACATCGGGGTTAGATTTCAATCGTGTAGCCTTATTATTAGCGGTTTTAGAAAAACGTGG
TAACCTAATGTTGCAAAATCAAGATGCATTTTTAACGGCAACAGGCGGAATTAAGTTAAATGAGCCAGCGATTGATTTGG
CAATCTGTATGGCTGTAGCTTCTAGTTATAAAAATAAAGAAATTTCTGCTACTGATTGTTTTGTAGGAGAGGTTGGTTTA
ACTGGTGAAATTAGAAGAGTTAATCAAATTGAAGCTAGAGTTAAAGAAGCTGCTAAGGTAGGCTTTAAGCGAATTTTTAT
CCCTAAACATAATTTAACTACTGAGCTTAAGAATAATTCTGCAATTGAAGTAATTGGTGTAGCAAGTCTGCCACAAGCTT
TAAAACTGGTTTTTAATTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)

AAA domain (AAA_25)

(68-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

59.825

99.782

0.597
  radA Streptococcus pneumoniae Rx1

59.825

99.782

0.597
  radA Streptococcus pneumoniae D39

59.825

99.782

0.597
  radA Streptococcus pneumoniae R6

59.825

99.782

0.597
  radA Streptococcus pneumoniae TIGR4

59.825

99.782

0.597
  radA Streptococcus mitis SK321

59.825

99.782

0.597
  radA Bacillus subtilis subsp. subtilis str. 168

55.921

99.346

0.556