Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LZG54_RS02595 Genome accession   NZ_CP090382
Coordinates   546956..547744 (+) Length   262 a.a.
NCBI ID   WP_411909640.1    Uniprot ID   -
Organism   Acinetobacter towneri strain SCLZS30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 541956..552744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZG54_RS02565 (LZG54_02570) - 542112..542537 (+) 426 WP_234114493.1 hypothetical protein -
  LZG54_RS02570 (LZG54_02575) - 542756..544039 (+) 1284 WP_234114494.1 glycosyltransferase family 4 protein -
  LZG54_RS02575 (LZG54_02580) - 544065..544616 (+) 552 WP_004975087.1 phosphatase PAP2 family protein -
  LZG54_RS02580 (LZG54_02585) iscX 544718..544918 (+) 201 WP_019836293.1 Fe-S cluster assembly protein IscX -
  LZG54_RS02585 (LZG54_02590) ndk 545080..545511 (+) 432 WP_004975092.1 nucleoside-diphosphate kinase -
  LZG54_RS02590 (LZG54_02595) rlmN 545648..546880 (+) 1233 WP_234114495.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  LZG54_RS02595 (LZG54_02600) pilF 546956..547744 (+) 789 WP_411909640.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LZG54_RS02600 (LZG54_02605) - 547735..548544 (+) 810 WP_004975098.1 helix-turn-helix domain-containing protein -
  LZG54_RS02605 (LZG54_02610) ispG 548567..549688 (+) 1122 WP_234114498.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LZG54_RS02610 (LZG54_02615) hisS 549692..550987 (+) 1296 WP_234114499.1 histidine--tRNA ligase -
  LZG54_RS02615 (LZG54_02620) - 551018..551719 (+) 702 WP_178318747.1 YfgM family protein -

Sequence

Protein


Download         Length: 262 a.a.        Molecular weight: 29437.31 Da        Isoelectric Point: 8.7703

>NTDB_id=641873 LZG54_RS02595 WP_411909640.1 546956..547744(+) (pilF) [Acinetobacter towneri strain SCLZS30]
MAQITTVCISAIFAAACQTTAPTLGQKDPEKAVQVRTQLAAEYLKNGDLDSAKRALDQALESDSRDSSANMMMGVLLQQE
GSKLNLEKADAYFKRAIAADPKNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKLGDIENAE
KSFKQALQANRDSYVSMLELSEILYLRQQFAPARQLYEQFVRAVGQKNQGARALWIGIRLARANGDQLGTQVLVNQLRAL
FPNSQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 789 bp        

>NTDB_id=641873 LZG54_RS02595 WP_411909640.1 546956..547744(+) (pilF) [Acinetobacter towneri strain SCLZS30]
ATCGCACAAATTACAACTGTTTGTATTTCGGCAATTTTTGCTGCAGCTTGTCAAACCACAGCTCCAACCTTAGGGCAAAA
AGACCCTGAAAAAGCGGTGCAAGTACGTACGCAGTTGGCAGCAGAATACTTAAAAAATGGCGATTTAGACTCTGCCAAAC
GTGCGTTGGATCAAGCTTTGGAAAGCGATTCACGTGATTCATCTGCTAATATGATGATGGGCGTGTTGCTGCAACAAGAA
GGCAGCAAGCTGAATTTAGAAAAAGCCGATGCGTATTTTAAGCGTGCCATTGCCGCTGACCCAAAAAATGCACAAGCGCG
TAACAACTATGGTACGTATTTATATCAAGTAGAACGCTATAATGATGCGATTGAGCAGCTCAGTATTGCAGGTGCAACAC
TGGGTTATGATCAACGCTTTAGAGCATTGGAAAATGTAGGTCGTATTTACTTGAAATTAGGCGATATAGAGAATGCCGAA
AAGTCATTCAAACAAGCCTTGCAAGCCAATCGCGATTCTTATGTTTCAATGTTAGAGTTGTCGGAAATTTTATATTTAAG
ACAACAATTTGCACCCGCGCGACAATTGTATGAACAATTTGTACGTGCTGTGGGGCAAAAAAATCAGGGTGCGCGTGCCT
TATGGATTGGGATTCGTTTAGCACGTGCCAATGGCGATCAATTGGGAACTCAGGTTTTAGTGAACCAGCTGCGTGCATTA
TTTCCCAATAGCCAAGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(39-73)

Tetratricopeptide repeat (TPR_16)

(147-203)

Tetratricopeptide repeat (TPR_17)

(93-125)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.381

96.183

0.744
  pilF Acinetobacter baylyi ADP1

72.441

96.947

0.702