Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   U722_RS01805 Genome accession   NC_023073
Coordinates   360702..361139 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens LFB112     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 355702..366139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U722_RS20115 - 356282..356407 (-) 126 Protein_304 hypothetical protein -
  U722_RS01775 (U722_01815) - 356560..357762 (+) 1203 WP_024084872.1 GTP-binding protein -
  U722_RS01780 (U722_01820) - 357827..358963 (+) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  U722_RS01785 (U722_01825) - 358978..359412 (+) 435 WP_021494203.1 RDD family protein -
  U722_RS01790 (U722_01830) - 359529..359804 (+) 276 WP_369719109.1 YckD family protein -
  U722_RS20400 - 359908..359991 (+) 84 WP_223151977.1 family 1 glycosylhydrolase -
  U722_RS01795 (U722_01840) - 360023..360241 (+) 219 Protein_310 family 1 glycosylhydrolase -
  U722_RS01800 (U722_01845) nin/comJ 360282..360680 (-) 399 WP_014304317.1 competence protein ComJ Regulator
  U722_RS01805 (U722_01850) nucA/comI 360702..361139 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  U722_RS01810 (U722_01855) hxlB 361498..362055 (-) 558 WP_003156587.1 6-phospho-3-hexuloisomerase -
  U722_RS01815 (U722_01860) hxlA 362052..362687 (-) 636 WP_024084873.1 3-hexulose-6-phosphate synthase -
  U722_RS01820 (U722_01865) - 362919..363281 (+) 363 WP_014304320.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=64166 U722_RS01805 WP_003156588.1 360702..361139(-) (nucA/comI) [Bacillus amyloliquefaciens LFB112]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=64166 U722_RS01805 WP_003156588.1 360702..361139(-) (nucA/comI) [Bacillus amyloliquefaciens LFB112]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment