Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LWM71_RS06495 Genome accession   NZ_CP090313
Coordinates   1263800..1265173 (-) Length   457 a.a.
NCBI ID   WP_003670547.1    Uniprot ID   A0AAW4X7I2
Organism   Limosilactobacillus reuteri strain DS0384     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1258800..1270173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LWM71_RS06465 (LWM71_06465) secE 1259265..1259441 (-) 177 WP_003666294.1 preprotein translocase subunit SecE -
  LWM71_RS06470 (LWM71_06470) rpmG 1259453..1259602 (-) 150 WP_003666293.1 50S ribosomal protein L33 -
  LWM71_RS06475 (LWM71_06475) rlmB 1259668..1260417 (-) 750 WP_003667343.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LWM71_RS06480 (LWM71_06480) - 1260407..1260820 (-) 414 WP_003670544.1 Mini-ribonuclease 3 -
  LWM71_RS06485 (LWM71_06485) cysS 1260832..1262244 (-) 1413 WP_035159778.1 cysteine--tRNA ligase -
  LWM71_RS06490 (LWM71_06490) - 1262625..1263782 (-) 1158 WP_003670546.1 PIN/TRAM domain-containing protein -
  LWM71_RS06495 (LWM71_06495) radA 1263800..1265173 (-) 1374 WP_003670547.1 DNA repair protein RadA Machinery gene
  LWM71_RS06500 (LWM71_06500) - 1265199..1265735 (-) 537 WP_003670548.1 hypothetical protein -
  LWM71_RS06505 (LWM71_06505) - 1265874..1266170 (+) 297 WP_003666282.1 GNAT family N-acetyltransferase -
  LWM71_RS06510 (LWM71_06510) rpiA 1266182..1266865 (+) 684 WP_003670550.1 ribose-5-phosphate isomerase RpiA -
  LWM71_RS06515 (LWM71_06515) - 1266894..1268234 (+) 1341 WP_003670551.1 aminopeptidase C -
  LWM71_RS06520 (LWM71_06520) - 1268342..1269133 (-) 792 WP_003670552.1 transporter substrate-binding domain-containing protein -
  LWM71_RS06525 (LWM71_06525) - 1269149..1269898 (-) 750 WP_003670553.1 amino acid ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49933.24 Da        Isoelectric Point: 8.4221

>NTDB_id=641474 LWM71_RS06495 WP_003670547.1 1263800..1265173(-) (radA) [Limosilactobacillus reuteri strain DS0384]
MAKIRTQYVCQNCGYNSPRYLGRCPNCGEWNTLVEEQVEASSAPTKKATTTLTGLVAKPQKINEIDSTETPRVKTKLNEL
NRVLGGGIVPGSLILIGGDPGIGKSTLLLQVSGQLSDEHHRVLYVSGEESGTQIKMRAERLKVAGDDFYVYPETNMDSIR
DTIRDLKPEYVVIDSVQTMQATDVSSAIGSVSQIREVTAQLMQIAKSNNITIFVVGHVTKGGAIAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMHTNGLTEVKNPSEIFLEERLHDATGSAVVVSLEGTRPILVEIQALVTPTVY
GNAQRTATGLSRNRVSLIMAVLEKRANLMLQNQDAYLKAAGGVKLDEPAIDLAIAVSIASSYRDKGTKPTDAFVGEVGLT
GEIRRVNRIEQRVAEAEKLGFKRIFIPKNNLKGWKPSTNIQVVGVSTLKEALYLALG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=641474 LWM71_RS06495 WP_003670547.1 1263800..1265173(-) (radA) [Limosilactobacillus reuteri strain DS0384]
ATGGCAAAAATTCGAACACAATATGTTTGCCAAAACTGTGGTTATAACTCACCACGCTATTTAGGCCGGTGTCCAAATTG
TGGTGAGTGGAATACTTTAGTAGAAGAACAGGTAGAAGCTAGTTCTGCACCAACCAAAAAAGCAACCACCACCTTAACCG
GCTTAGTTGCCAAACCACAGAAAATTAATGAGATTGATAGTACAGAAACTCCCCGTGTTAAAACAAAATTGAACGAGTTA
AATCGCGTTTTAGGTGGAGGAATCGTTCCCGGATCACTAATTCTAATTGGAGGAGATCCGGGAATCGGAAAATCGACTCT
CCTTTTACAAGTTTCCGGACAATTAAGTGATGAGCATCACCGGGTTTTATATGTGTCTGGAGAAGAAAGTGGGACACAAA
TTAAAATGCGGGCGGAACGATTAAAAGTTGCTGGGGATGACTTTTATGTATATCCAGAAACGAATATGGATAGTATTCGG
GATACTATTCGTGACCTCAAGCCAGAGTATGTCGTGATTGACTCGGTACAAACGATGCAGGCGACTGATGTAAGTTCAGC
AATTGGTAGTGTATCGCAAATTCGTGAAGTTACTGCCCAGTTAATGCAAATTGCTAAAAGTAATAACATTACGATTTTTG
TCGTTGGACATGTGACAAAGGGCGGTGCAATCGCTGGTCCAAAGATCTTGGAGCACATGGTTGATACAGTCTTGTATTTT
GAAGGAGACCTGCACCATACATATCGTATTTTGCGATCAGTGAAAAATCGATTTGGTTCAACTAACGAGCTTGGTATTTT
TGAAATGCATACAAATGGGCTGACGGAAGTAAAAAATCCATCAGAAATCTTTTTAGAGGAACGATTACATGATGCAACCG
GTTCAGCGGTGGTTGTTTCATTAGAAGGAACACGACCAATTTTAGTTGAAATTCAAGCGCTGGTAACACCGACCGTTTAT
GGGAATGCACAGCGAACTGCTACCGGTTTGAGCCGAAACCGAGTATCATTGATAATGGCGGTATTAGAGAAACGTGCTAA
CCTAATGCTTCAAAATCAAGATGCTTACTTAAAAGCAGCTGGGGGCGTTAAGTTAGATGAGCCAGCAATTGATTTGGCAA
TTGCAGTAAGTATTGCCTCCAGTTATCGGGATAAAGGAACCAAGCCCACCGATGCATTTGTCGGTGAGGTAGGTTTGACT
GGGGAAATTCGCCGGGTAAACCGAATTGAACAGCGGGTTGCTGAAGCAGAAAAACTTGGCTTTAAGCGAATCTTTATTCC
CAAAAATAATTTAAAGGGTTGGAAACCGTCAACTAATATTCAAGTAGTCGGTGTCTCAACGCTTAAAGAGGCGTTATATT
TAGCGCTGGGGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(65-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

66.372

98.906

0.656
  radA Streptococcus mitis NCTC 12261

66.372

98.906

0.656
  radA Streptococcus pneumoniae Rx1

66.15

98.906

0.654
  radA Streptococcus pneumoniae D39

66.15

98.906

0.654
  radA Streptococcus pneumoniae R6

66.15

98.906

0.654
  radA Streptococcus pneumoniae TIGR4

66.15

98.906

0.654
  radA Bacillus subtilis subsp. subtilis str. 168

62.801

100

0.628