Detailed information NTG0064146

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: T223_RS24870
• Genome accession: NC_023066
• Coordinates: 5355117..5355989 (+)
• Length: 290 a.a.
• NCBI ID: WP_003107301.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa LES431
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5350117..5360989

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
T223_RS24855 (T223_25050)	-	5351500..5351964 (-)	465	WP_003122079.1	pilin	-
T223_RS24860 (T223_25055)	pilB	5352195..5353895 (+)	1701	WP_003122078.1	type IV-A pilus assembly ATPase PilB	Machinery gene
T223_RS24865 (T223_25060)	pilC	5353899..5355116 (+)	1218	WP_003107299.1	type II secretion system F family protein	Machinery gene
T223_RS24870 (T223_25065)	pilD	5355117..5355989 (+)	873	WP_003107301.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
T223_RS24875 (T223_25070)	coaE	5355986..5356597 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
T223_RS24880 (T223_25075)	yacG	5356594..5356794 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
T223_RS24885 (T223_25080)	-	5356831..5357040 (-)	210	WP_003094660.1	hypothetical protein	-
T223_RS24890 (T223_25085)	-	5357146..5357835 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
T223_RS24895 (T223_25090)	-	5357832..5358302 (-)	471	WP_003103866.1	hypothetical protein	-
T223_RS24900 (T223_25095)	-	5358299..5358724 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
T223_RS24905 (T223_25100)	-	5358857..5359486 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
T223_RS24910 (T223_25105)	-	5359483..5359932 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
T223_RS24915 (T223_25110)	-	5359958..5360128 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31873.90 Da
• Isoelectric Point: 7.7068

>NTDB_id=64146 T223_RS24870 WP_003107301.1 5355117..5355989(+) (pilD) [Pseudomonas aeruginosa LES431]
MTLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=64146 T223_RS24870 WP_003107301.1 5355117..5355989(+) (pilD) [Pseudomonas aeruginosa LES431]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCAAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	48.921	95.862	0.469