Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   OGM43_RS18965 Genome accession   NZ_CP107147
Coordinates   3891655..3892095 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain R14     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3886655..3897095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OGM43_RS18940 (OGM43_18910) coaE 3887145..3887765 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  OGM43_RS18945 (OGM43_18915) - 3887790..3887834 (+) 45 WP_120795372.1 protein YacM -
  OGM43_RS18950 (OGM43_18920) guaC 3887990..3889033 (+) 1044 WP_001217320.1 GMP reductase -
  OGM43_RS18955 (OGM43_18925) hofC 3889068..3890270 (-) 1203 WP_000157289.1 protein transport protein HofC -
  OGM43_RS18960 (OGM43_18930) gspE 3890260..3891645 (-) 1386 Protein_3714 type II secretion system protein GspE -
  OGM43_RS18965 (OGM43_18935) pilA 3891655..3892095 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  OGM43_RS18970 (OGM43_18940) nadC 3892299..3893192 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OGM43_RS18975 (OGM43_18945) ampD 3893280..3893831 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OGM43_RS18980 (OGM43_18950) ampE 3893828..3894682 (+) 855 WP_000171980.1 beta-lactamase regulator AmpE -
  OGM43_RS18985 (OGM43_18955) aroP 3894725..3896095 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=641450 OGM43_RS18965 WP_000360914.1 3891655..3892095(-) (pilA) [Escherichia coli strain R14]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=641450 OGM43_RS18965 WP_000360914.1 3891655..3892095(-) (pilA) [Escherichia coli strain R14]
ATGGACAAGCAACGCGGTTTTACACTTATTGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363