Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LVJ09_RS00975 Genome accession   NZ_CP090216
Coordinates   196895..198265 (+) Length   456 a.a.
NCBI ID   WP_168413290.1    Uniprot ID   A0AAE9XII1
Organism   Vagococcus lutrae strain VF20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 191895..203265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ09_RS00950 (LVJ09_00945) - 193075..193794 (+) 720 WP_274981703.1 pseudouridine synthase -
  LVJ09_RS00955 (LVJ09_00950) - 193908..194222 (+) 315 WP_023607060.1 rhodanese-like domain-containing protein -
  LVJ09_RS00960 (LVJ09_00955) - 194223..195869 (+) 1647 WP_274981704.1 FAD-dependent oxidoreductase -
  LVJ09_RS00965 (LVJ09_00960) - 195884..196141 (+) 258 WP_126762350.1 metal-sensing transcriptional repressor -
  LVJ09_RS00970 (LVJ09_00965) - 196257..196790 (+) 534 WP_126762352.1 dUTP diphosphatase -
  LVJ09_RS00975 (LVJ09_00970) radA 196895..198265 (+) 1371 WP_168413290.1 DNA repair protein RadA Machinery gene
  LVJ09_RS00980 (LVJ09_00975) - 198284..199414 (+) 1131 WP_274981705.1 PIN/TRAM domain-containing protein -
  LVJ09_RS00985 (LVJ09_00980) gltX 199510..200976 (+) 1467 WP_023607066.1 glutamate--tRNA ligase -
  LVJ09_RS00990 (LVJ09_00985) epsC 201231..201788 (+) 558 WP_023607067.1 serine O-acetyltransferase EpsC -
  LVJ09_RS00995 (LVJ09_00990) cysS 201772..203190 (+) 1419 WP_274981706.1 cysteine--tRNA ligase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50116.58 Da        Isoelectric Point: 6.1973

>NTDB_id=641231 LVJ09_RS00975 WP_168413290.1 196895..198265(+) (radA) [Vagococcus lutrae strain VF20]
MAKKQRTQFECQACGYISPKYLGRCPNCGAWNEMMEVKIQEEPDRHSRVSLAGKRTTAKRLTEVAMEKEPRVKTQMDELN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSQQLNQADGVVLYVSGEESAEQIKMRAERLGIKGNDFYIYPETDMSQIRQ
TIEELQPDYVIIDSIQTMMQPEIASASGSVSQVRETTAELMQIAKTNHIAIFIVGHVTKEGALAGPRMLEHMVDTVLYFE
GDRHHTFRILRAVKNRFGSTNEIGIFEMVESGLQEVLNPSEVFLEERLDGATGSAIVVSMEGSRPILAEVQALVSPTLFG
NAKRTATGLDYNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLDEPAIDLAIAMSIASSYKDTGTSATECFIGEIGLTG
EVRRVNRIDQRIREAQKLGFKKVYVPKNNLHGYTPPADIEVVGIATLSEALRKVFP

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=641231 LVJ09_RS00975 WP_168413290.1 196895..198265(+) (radA) [Vagococcus lutrae strain VF20]
GTGGCGAAAAAGCAACGTACACAATTTGAATGTCAAGCATGCGGTTACATTTCTCCTAAATATTTAGGTAGATGCCCTAA
CTGCGGTGCTTGGAATGAAATGATGGAAGTGAAGATACAAGAAGAACCTGACCGTCATAGTCGCGTCAGCCTAGCTGGTA
AGCGAACAACGGCTAAGCGATTAACAGAAGTGGCGATGGAAAAAGAACCCCGTGTAAAAACACAAATGGACGAGCTTAAT
CGTGTGTTGGGTGGTGGCGTTGTTCCCGGGTCACTTGTTTTAATTGGTGGAGACCCTGGGATTGGGAAATCTACGTTGTT
ACTACAAGTTTCGCAACAGCTTAATCAAGCTGATGGCGTAGTTTTATACGTGTCCGGTGAGGAAAGTGCCGAGCAGATAA
AAATGCGTGCGGAGCGTTTAGGGATTAAAGGCAATGATTTTTATATTTATCCAGAAACAGATATGTCTCAGATACGTCAA
ACTATCGAGGAATTACAACCAGACTATGTCATCATTGATTCCATCCAGACGATGATGCAACCGGAGATAGCCAGTGCGTC
AGGTAGTGTGAGTCAAGTCCGAGAAACGACTGCTGAGTTGATGCAAATCGCTAAAACTAATCATATTGCTATTTTTATCG
TAGGCCATGTGACAAAGGAAGGCGCCTTAGCAGGACCTAGAATGTTAGAGCATATGGTAGATACGGTGTTGTATTTTGAA
GGGGATCGTCATCATACCTTTCGAATTTTGCGAGCAGTTAAAAACCGTTTTGGATCGACAAATGAGATTGGGATATTTGA
GATGGTGGAGTCGGGCTTACAGGAAGTCTTAAATCCTTCTGAAGTCTTCTTAGAAGAGCGATTAGACGGTGCGACTGGCT
CGGCGATTGTGGTGTCGATGGAAGGCAGTCGTCCGATTTTAGCAGAAGTGCAAGCGCTTGTATCTCCTACTTTATTTGGA
AACGCAAAACGGACCGCGACTGGGTTAGATTATAACCGGGTGTCACTGATTATGGCAGTGTTAGAAAAACGAGCCGGTCT
TTTGTTGCAAAATCAAGATGCTTATTTAAAAGCTGCTGGTGGTGTTAAGCTAGATGAACCGGCGATTGATTTAGCCATTG
CGATGAGTATCGCTTCGAGCTACAAAGATACGGGAACTAGTGCAACCGAGTGTTTTATTGGCGAAATTGGTTTGACCGGT
GAAGTGCGACGGGTGAATCGGATTGATCAGCGCATTAGAGAAGCTCAAAAGTTAGGCTTTAAAAAAGTGTACGTACCAAA
AAACAATTTACATGGATATACACCGCCAGCTGATATTGAAGTGGTTGGAATTGCCACCTTATCAGAAGCGTTAAGAAAAG
TTTTTCCTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-34)

AAA domain (AAA_25)

(71-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.066

99.781

0.739
  radA Streptococcus mitis SK321

74.066

99.781

0.739
  radA Streptococcus pneumoniae Rx1

73.846

99.781

0.737
  radA Streptococcus pneumoniae D39

73.846

99.781

0.737
  radA Streptococcus pneumoniae R6

73.846

99.781

0.737
  radA Streptococcus pneumoniae TIGR4

73.846

99.781

0.737
  radA Bacillus subtilis subsp. subtilis str. 168

66.15

99.123

0.656