Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LXM48_RS01575 Genome accession   NZ_CP090180
Coordinates   305612..306412 (+) Length   266 a.a.
NCBI ID   WP_234304488.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain DJ-Red     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 300612..311412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LXM48_RS01545 (LXM48_01545) - 300824..301240 (+) 417 WP_004983505.1 hypothetical protein -
  LXM48_RS01550 (LXM48_01550) - 301381..302661 (+) 1281 WP_205667599.1 glycosyltransferase family 1 protein -
  LXM48_RS01555 (LXM48_01555) - 302854..303405 (+) 552 WP_058870303.1 phosphatase PAP2 family protein -
  LXM48_RS01560 (LXM48_01560) iscX 303495..303695 (+) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  LXM48_RS01565 (LXM48_01565) ndk 303806..304237 (+) 432 WP_004983508.1 nucleoside-diphosphate kinase -
  LXM48_RS01570 (LXM48_01570) rlmN 304360..305595 (+) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  LXM48_RS01575 (LXM48_01575) pilF 305612..306412 (+) 801 WP_234304488.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LXM48_RS01580 (LXM48_01580) - 306403..307179 (+) 777 WP_151836404.1 helix-turn-helix domain-containing protein -
  LXM48_RS01585 (LXM48_01585) ispG 307198..308313 (+) 1116 WP_004697677.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LXM48_RS01590 (LXM48_01590) hisS 308310..309605 (+) 1296 WP_234304489.1 histidine--tRNA ligase -
  LXM48_RS01595 (LXM48_01595) - 309633..310331 (+) 699 WP_234304490.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29987.06 Da        Isoelectric Point: 9.3364

>NTDB_id=641011 LXM48_RS01575 WP_234304488.1 305612..306412(+) (pilF) [Acinetobacter johnsonii strain DJ-Red]
MKKPVSKLCLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLRVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYVQFVRAVGEKNLDARALWIGIRIARASHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=641011 LXM48_RS01575 WP_234304488.1 305612..306412(+) (pilF) [Acinetobacter johnsonii strain DJ-Red]
TTGAAAAAACCCGTTTCTAAACTTTGCCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGATCCAAGTAAAGCAGTACAAGTTAGAACGCAGTTGGCGGCTGAATATATTAAGTCGAATGATTATG
ATGCTGCGAAACGTACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTGTTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCAGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCGAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCTGCTTTTAAACAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGTACAGTTTGTGCGAGCGGTTGGCGAGAAAAACTTAG
ATGCACGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAGTCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
CTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-201)

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_19)

(89-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.284

100

0.688
  pilF Acinetobacter baylyi ADP1

63.91

100

0.639