Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   OGM52_RS20445 Genome accession   NZ_CP107120
Coordinates   4135682..4136122 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain S12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4130682..4141122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OGM52_RS20425 (OGM52_20405) aroP 4131682..4133052 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  OGM52_RS20430 (OGM52_20410) ampE 4133095..4133949 (-) 855 WP_000171980.1 beta-lactamase regulator AmpE -
  OGM52_RS20435 (OGM52_20415) ampD 4133946..4134497 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OGM52_RS20440 (OGM52_20420) nadC 4134585..4135478 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OGM52_RS20445 (OGM52_20425) pilA 4135682..4136122 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  OGM52_RS20450 (OGM52_20430) gspE 4136132..4137517 (+) 1386 Protein_4031 type II secretion system protein GspE -
  OGM52_RS20455 (OGM52_20435) hofC 4137507..4138709 (+) 1203 WP_000157289.1 protein transport protein HofC -
  OGM52_RS20460 (OGM52_20440) guaC 4138744..4139787 (-) 1044 WP_001217320.1 GMP reductase -
  OGM52_RS20465 (OGM52_20445) - 4139943..4139987 (-) 45 WP_120795372.1 protein YacM -
  OGM52_RS20470 (OGM52_20450) coaE 4140012..4140632 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=640676 OGM52_RS20445 WP_000360914.1 4135682..4136122(+) (pilA) [Escherichia coli strain S12]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=640676 OGM52_RS20445 WP_000360914.1 4135682..4136122(+) (pilA) [Escherichia coli strain S12]
ATGGACAAGCAACGCGGTTTTACACTTATTGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363