Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LXE94_RS00580 Genome accession   NZ_CP090125
Coordinates   105681..107057 (+) Length   458 a.a.
NCBI ID   WP_257986363.1    Uniprot ID   -
Organism   Bacillus subtilis strain Bs96     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 100681..112057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LXE94_RS00560 (LXE94_00560) ctsR 101034..101498 (+) 465 WP_003225724.1 transcriptional regulator CtsR -
  LXE94_RS00565 (LXE94_00565) mcsA 101512..102069 (+) 558 WP_014475591.1 protein-arginine kinase activator protein McsA -
  LXE94_RS00570 (LXE94_00570) mcsB 102069..103160 (+) 1092 WP_257986362.1 protein arginine kinase -
  LXE94_RS00575 (LXE94_00575) clpC 103157..105589 (+) 2433 WP_014475592.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  LXE94_RS00580 (LXE94_00580) radA 105681..107057 (+) 1377 WP_257986363.1 DNA repair protein RadA Machinery gene
  LXE94_RS00585 (LXE94_00585) disA 107061..108143 (+) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  LXE94_RS00590 (LXE94_00590) yacL 108259..109359 (+) 1101 WP_017695823.1 PIN/TRAM domain-containing protein -
  LXE94_RS00595 (LXE94_00595) ispD 109374..110072 (+) 699 WP_041332619.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  LXE94_RS00600 (LXE94_00600) ispF 110065..110541 (+) 477 WP_014475594.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49454.94 Da        Isoelectric Point: 8.2399

>NTDB_id=640662 LXE94_RS00580 WP_257986363.1 105681..107057(+) (radA) [Bacillus subtilis strain Bs96]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVSEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=640662 LXE94_RS00580 WP_257986363.1 105681..107057(+) (radA) [Bacillus subtilis strain Bs96]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATTGGCGGTGATCCCGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTCCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGTCTCGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCGGC
TCCAGGCAGTGTGTCACAGGTCAGAGAGTGTACCGCTGAGCTGATGAAAATTGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAAGGGTCTATTGCAGGTCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATTTT
CGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCGGAAATTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAG
GCTCGAGTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCGCTCATCTCGCCAACAAGTTTT
GGCAACCCAAGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCTCTGTTAATGGCTGTGTTAGAAAAAAGAGTAGG
GCTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCCA
TTGCGATTAGTATTGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGTTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGTTTTAAACGCATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTTCAGAAGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.345

100

0.993
  radA Streptococcus pneumoniae Rx1

63.355

98.908

0.627
  radA Streptococcus pneumoniae D39

63.355

98.908

0.627
  radA Streptococcus pneumoniae R6

63.355

98.908

0.627
  radA Streptococcus pneumoniae TIGR4

63.355

98.908

0.627
  radA Streptococcus mitis NCTC 12261

63.355

98.908

0.627
  radA Streptococcus mitis SK321

63.135

98.908

0.624