Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LW858_RS18455 Genome accession   NZ_CP090081
Coordinates   3633590..3634024 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus cereus strain CPT56D-587-MTF     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3628590..3639024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LW858_RS18440 (LW858_18440) - 3629766..3630323 (+) 558 WP_139846722.1 PadR family transcriptional regulator -
  LW858_RS18445 (LW858_18445) metE 3630837..3633125 (+) 2289 WP_001007608.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  LW858_RS18450 (LW858_18450) comJ 3633164..3633553 (-) 390 WP_000424030.1 competence protein ComJ -
  LW858_RS18455 (LW858_18455) nucA/comI 3633590..3634024 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  LW858_RS18460 (LW858_18460) - 3634251..3635000 (+) 750 WP_233936591.1 DUF3967 domain-containing protein -
  LW858_RS18465 (LW858_18465) kinB 3635039..3636313 (-) 1275 WP_000420370.1 sporulation sensor histidine kinase KinB -
  LW858_RS18470 (LW858_18470) - 3636624..3636824 (+) 201 WP_000929278.1 hypothetical protein -
  LW858_RS18475 (LW858_18475) - 3636947..3638110 (+) 1164 WP_233935245.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=640607 LW858_RS18455 WP_000811494.1 3633590..3634024(-) (nucA/comI) [Bacillus cereus strain CPT56D-587-MTF]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=640607 LW858_RS18455 WP_000811494.1 3633590..3634024(-) (nucA/comI) [Bacillus cereus strain CPT56D-587-MTF]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGTTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549