Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LXF01_RS13645 Genome accession   NZ_CP090067
Coordinates   2789611..2790414 (-) Length   267 a.a.
NCBI ID   WP_078189612.1    Uniprot ID   -
Organism   Acinetobacter sp. SH20PTE14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2784611..2795414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LXF01_RS13625 (LXF01_13625) - 2785653..2786351 (-) 699 WP_078189608.1 tetratricopeptide repeat protein -
  LXF01_RS13630 (LXF01_13630) hisS 2786382..2787677 (-) 1296 WP_078189609.1 histidine--tRNA ligase -
  LXF01_RS13635 (LXF01_13635) ispG 2787681..2788802 (-) 1122 WP_233944548.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LXF01_RS13640 (LXF01_13640) - 2788826..2789620 (-) 795 WP_180124989.1 RodZ domain-containing protein -
  LXF01_RS13645 (LXF01_13645) pilF 2789611..2790414 (-) 804 WP_078189612.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LXF01_RS13650 (LXF01_13650) rlmN 2790430..2791665 (-) 1236 WP_233944549.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  LXF01_RS13655 (LXF01_13655) ndk 2791789..2792220 (-) 432 WP_078189614.1 nucleoside-diphosphate kinase -
  LXF01_RS13660 (LXF01_13660) iscX 2792330..2792530 (-) 201 WP_078189615.1 Fe-S cluster assembly protein IscX -
  LXF01_RS13665 (LXF01_13665) - 2792652..2793203 (-) 552 WP_233944550.1 phosphatase PAP2 family protein -
  LXF01_RS13670 (LXF01_13670) - 2793396..2794670 (-) 1275 WP_233944551.1 glycosyltransferase family 1 protein -
  LXF01_RS13675 (LXF01_13675) - 2794870..2795091 (-) 222 WP_233944552.1 ATP-binding protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 30101.19 Da        Isoelectric Point: 9.2661

>NTDB_id=640552 LXF01_RS13645 WP_078189612.1 2789611..2790414(-) (pilF) [Acinetobacter sp. SH20PTE14]
MKKLTYKFTFVSALCASVLALTACQTPEIGKKDPEKAVKIRTQLAVEHLKAGDLDSAKRALDQALEVDSRDSQANMMMGV
LLQREGSPANLEKADSYFKRAISADSKNSQARNNYGTYLYQVERYNDAIEQFSIAGSTLGYDQRFKALENLGRIYLKVGD
VANAEKSFKQALQANRDSYVSMLELSEIFYLNQQFPAATQMYEQFVRAVGQKNQGARALWIGIRTARANGDNLGMQVLVN
QLRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=640552 LXF01_RS13645 WP_078189612.1 2789611..2790414(-) (pilF) [Acinetobacter sp. SH20PTE14]
TTGAAAAAGTTAACGTATAAGTTCACTTTCGTTTCAGCTTTATGTGCATCGGTTTTGGCATTAACAGCATGTCAAACGCC
TGAGATTGGGAAAAAAGACCCAGAGAAAGCCGTAAAAATCAGAACGCAATTGGCTGTTGAACACCTGAAAGCAGGGGATT
TGGACTCTGCTAAACGTGCATTAGATCAGGCTTTAGAGGTGGATTCTCGCGATTCCCAAGCCAATATGATGATGGGTGTC
TTACTACAACGAGAAGGCAGTCCAGCCAACTTAGAAAAAGCAGATAGTTATTTTAAGCGTGCAATTTCAGCAGACTCAAA
AAATTCTCAAGCACGTAACAACTACGGTACGTATTTATACCAAGTTGAGCGTTATAATGATGCGATTGAACAGTTCAGCA
TCGCAGGTTCAACTTTAGGTTATGATCAACGTTTCAAAGCATTGGAAAATTTAGGTCGAATTTACCTGAAAGTGGGTGAT
GTGGCGAATGCTGAAAAATCATTCAAACAAGCTTTGCAGGCTAATCGTGATTCCTATGTTTCAATGTTAGAGTTGTCTGA
AATCTTTTATTTGAATCAGCAGTTCCCTGCTGCCACACAAATGTATGAACAATTTGTACGTGCAGTAGGGCAAAAAAATC
AGGGTGCGCGTGCACTCTGGATTGGCATTCGAACAGCGCGTGCCAATGGCGACAATTTGGGTATGCAAGTACTGGTTAAC
CAGTTACGTGCTTTGTTCCCAGAAAGTCCTGAATACCAACGCTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(90-133)

Tetratricopeptide repeat (TPR_16)

(43-82)

Tetratricopeptide repeat (TPR_8)

(146-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.599

96.255

0.757
  pilF Acinetobacter baylyi ADP1

69.663

100

0.697