Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PALA20_RS05500 Genome accession   NZ_CP107064
Coordinates   1143476..1144858 (-) Length   460 a.a.
NCBI ID   WP_023127273.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1138476..1149858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA20_RS05465 (PALA20_01083) thiS 1138710..1138910 (-) 201 WP_003084514.1 sulfur carrier protein ThiS -
  PALA20_RS05470 (PALA20_01084) - 1139013..1139390 (-) 378 WP_003084513.1 DUF423 domain-containing protein -
  PALA20_RS05475 (PALA20_01085) mtgA 1139427..1140158 (+) 732 WP_003084512.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  PALA20_RS05480 (PALA20_01086) - 1140163..1140768 (-) 606 WP_003084511.1 hypothetical protein -
  PALA20_RS05485 (PALA20_01087) rpoH 1140833..1141687 (-) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  PALA20_RS05490 (PALA20_01088) ftsX 1141801..1142808 (-) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  PALA20_RS05495 (PALA20_01089) ftsE 1142808..1143479 (-) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  PALA20_RS05500 (PALA20_01090) pilA 1143476..1144858 (-) 1383 WP_023127273.1 signal recognition particle-docking protein FtsY Machinery gene
  PALA20_RS05505 (PALA20_01091) - 1144979..1146376 (+) 1398 WP_003084498.1 M16 family metallopeptidase -
  PALA20_RS05510 (PALA20_01092) - 1146369..1147856 (+) 1488 WP_003084496.1 M16 family metallopeptidase -
  PALA20_RS05515 (PALA20_01093) rsmD 1147856..1148452 (+) 597 WP_003084492.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  PALA20_RS05520 (PALA20_01094) - 1148455..1149021 (+) 567 WP_003084491.1 hypothetical protein -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 48945.39 Da        Isoelectric Point: 4.4808

>NTDB_id=640360 PALA20_RS05500 WP_023127273.1 1143476..1144858(-) (pilA) [Pseudomonas aeruginosa strain PALA20]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPTDQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPAEPAEP
PAPVAEAPLAGDEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQ
GLSKTSASIGEGMASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLR
PVEQPLQVDVAREPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGAD
SASVIFDAVQAAKARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVE
LTGLALTKLDGTAKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=640360 PALA20_RS05500 WP_023127273.1 1143476..1144858(-) (pilA) [Pseudomonas aeruginosa strain PALA20]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTACCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGACGTGGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGGCCGAGCCG
CCGGCGCCGGTTGCCGAAGCGCCGCTCGCCGGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGCCCCGAGGCGCC
CGTCGCACAACCCGAGCCTATCCTCGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCG
AGCCCGTTGCGCCGCTGGCCGCCGCTCCGGCAGTCAGCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAG
GGACTGTCCAAGACCAGCGCCAGCATCGGCGAAGGCATGGCCAGCCTGTTCCTCGGCCGCAAGGAAATCGACGACGACCT
GCTCGACGACATCGAGACGCGCCTGCTGACCGCCGACGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCA
AGCGCGTGGCGCGCAAGGAGCTGGCCGACAGCGGCGCGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGC
CCGGTGGAGCAGCCGTTGCAGGTCGACGTCGCGCGCGAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAA
GACCACCACCATCGGCAAGCTGGCGAAGAAGCTGCAACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCC
GCGCCGCCGCCGTGGAGCAGTTGCAGGTCTGGGGCGAGCGCAACCGCATCCCGGTGATCGCCCAGCACACTGGCGCCGAC
TCCGCCTCGGTGATCTTCGACGCGGTGCAGGCCGCCAAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACG
CCTGCATACCAAGGACAACCTGATGGAGGAGCTGAAGAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCC
ATGAGGTCCTGCTGGTGCTCGACGCCGGCACCGGGCAGAACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAG
CTGACCGGCCTGGCCCTGACCAAACTGGACGGCACCGCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCT
GCCGATCCGCTATATCGGTGTCGGCGAGGGGATCGACGATCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCT
TCGCAGAGCGGGAGAACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(170-235)

SRP54-type protein, GTPase domain (SRP54)

(255-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

54.354

72.391

0.393