Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LS684_RS00580 Genome accession   NZ_CP089997
Coordinates   112895..114274 (+) Length   459 a.a.
NCBI ID   WP_233809027.1    Uniprot ID   -
Organism   Cytobacillus spongiae strain CY-G     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 107895..119274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LS684_RS00560 (LS684_00560) - 108068..108529 (+) 462 WP_233809023.1 CtsR family transcriptional regulator -
  LS684_RS00565 (LS684_00565) - 108552..109100 (+) 549 WP_233809024.1 UvrB/UvrC motif-containing protein -
  LS684_RS00570 (LS684_00570) - 109103..110173 (+) 1071 WP_233809025.1 protein arginine kinase -
  LS684_RS00575 (LS684_00575) clpC 110187..112628 (+) 2442 WP_233809026.1 ATP-dependent protease ATP-binding subunit ClpC -
  LS684_RS00580 (LS684_00580) radA 112895..114274 (+) 1380 WP_233809027.1 DNA repair protein RadA Machinery gene
  LS684_RS00585 (LS684_00585) disA 114278..115351 (+) 1074 WP_233809028.1 DNA integrity scanning diadenylate cyclase DisA -
  LS684_RS00590 (LS684_00590) - 115579..116661 (+) 1083 WP_233809029.1 PIN/TRAM domain-containing protein -
  LS684_RS00595 (LS684_00595) ispD 116681..117388 (+) 708 WP_233809030.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  LS684_RS00600 (LS684_00600) ispF 117391..117873 (+) 483 WP_233809031.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49946.43 Da        Isoelectric Point: 7.4340

>NTDB_id=639891 LS684_RS00580 WP_233809027.1 112895..114274(+) (radA) [Cytobacillus spongiae strain CY-G]
MAKRKTKFMCQDCGYESPKWMGKCPGCGQWNTMVEEIEKTAPARKGAFVHSQSTGVASKATPITSIETVSEPRMETDLKE
LNRVLGGGVVRGSLVLIGGDPGIGKSTLLLQVSSQLANKQQKVLYISGEESMRQTKLRADRLSVTSENLLIYSETNLEEI
NLTIEQVNPQFVIVDSIQTIFHPHVTSAPGSVSQVRECTAELMRIGKTKGIAIFIVGHVTKEGSIAGPRLLEHMVDTVLY
FEGERHHSYRILRAVKNRFGSTNEMGIFEMKELGLEEVENPSEIFLEERSQGASGSTVVASMEGTRPVLVEIQALISPTS
FGNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFRDQPSKATDCIIGEVGL
TGEVRRVSRIEQRVQEAAKLGFERVILPANNLGGWKGPRGIELVGVHSVSEALKATLGG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=639891 LS684_RS00580 WP_233809027.1 112895..114274(+) (radA) [Cytobacillus spongiae strain CY-G]
ATGGCCAAACGAAAAACGAAATTTATGTGTCAAGATTGTGGGTATGAATCACCAAAATGGATGGGAAAATGTCCAGGATG
TGGTCAATGGAACACAATGGTGGAAGAAATAGAAAAGACAGCTCCAGCAAGGAAAGGAGCTTTTGTTCACTCGCAATCCA
CTGGAGTTGCTTCGAAAGCAACACCTATTACATCAATTGAAACTGTTAGTGAGCCGAGGATGGAAACAGACTTGAAAGAA
CTGAATCGAGTGCTTGGTGGAGGAGTCGTTAGAGGTTCTTTAGTACTGATTGGCGGGGACCCTGGTATAGGAAAATCGAC
ATTGTTGCTGCAAGTGTCTTCTCAATTAGCGAATAAACAGCAAAAGGTGTTATATATATCTGGAGAAGAATCAATGCGAC
AAACCAAGCTTAGAGCGGATCGATTAAGTGTCACCTCAGAAAATCTCCTCATCTATTCAGAAACCAACTTGGAAGAAATT
AATTTGACGATTGAACAGGTTAACCCTCAATTTGTTATTGTGGATTCAATACAAACGATATTTCATCCACATGTAACGTC
AGCTCCTGGAAGTGTGTCTCAAGTAAGAGAATGTACAGCAGAGCTTATGAGGATTGGAAAAACAAAGGGAATTGCCATCT
TTATTGTCGGGCATGTTACAAAAGAAGGGTCGATTGCCGGTCCTAGATTACTAGAGCATATGGTGGACACAGTTCTATAT
TTTGAAGGAGAGCGTCACCATTCCTATCGAATATTGCGAGCGGTGAAGAATCGATTTGGATCAACCAATGAGATGGGGAT
CTTTGAGATGAAGGAGCTTGGATTAGAGGAAGTTGAAAACCCATCGGAAATTTTCTTAGAGGAGCGTTCCCAAGGTGCAT
CTGGTTCCACTGTCGTTGCATCAATGGAAGGAACACGTCCTGTCCTCGTTGAAATTCAAGCGTTAATCTCGCCAACTAGC
TTTGGAAACCCACGGAGAATGGCTACAGGAATAGATCATAATCGAGTATCGTTACTTATGGCTGTATTAGAAAAGCGGGT
AGGATTATTACTTCAAAACCAAGATGCCTATTTAAAGGTAGCTGGTGGAGTAAAACTGGATGAACCCGCCATTGACCTAG
CTATTGCAGTTAGCATCGCCTCTAGCTTTCGAGATCAGCCGTCTAAGGCGACAGATTGCATAATTGGGGAAGTAGGTTTA
ACCGGTGAAGTTCGAAGAGTTTCACGCATTGAGCAGAGAGTGCAGGAAGCTGCCAAGCTAGGTTTTGAACGAGTCATTTT
ACCAGCAAATAATCTTGGTGGATGGAAAGGACCTAGAGGGATTGAATTAGTGGGAGTTCACTCAGTCAGTGAGGCATTGA
AGGCAACATTAGGGGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(366-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

77.391

100

0.776
  radA Streptococcus pneumoniae Rx1

61.454

98.911

0.608
  radA Streptococcus pneumoniae D39

61.454

98.911

0.608
  radA Streptococcus pneumoniae R6

61.454

98.911

0.608
  radA Streptococcus pneumoniae TIGR4

61.454

98.911

0.608
  radA Streptococcus mitis NCTC 12261

61.454

98.911

0.608
  radA Streptococcus mitis SK321

61.454

98.911

0.608