Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LU301_RS07840 Genome accession   NZ_CP089978
Coordinates   1577141..1577965 (-) Length   274 a.a.
NCBI ID   WP_305274023.1    Uniprot ID   -
Organism   Moraxella sp. ZY210820     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1572141..1582965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LU301_RS07820 (LU301_07805) - 1573215..1574105 (-) 891 WP_305269482.1 hypothetical protein -
  LU301_RS07825 (LU301_07810) - 1574165..1575055 (-) 891 WP_305269483.1 cation transporter -
  LU301_RS07830 (LU301_07815) ispG 1575090..1576205 (-) 1116 WP_305269486.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LU301_RS07835 (LU301_07820) - 1576353..1577150 (-) 798 WP_305269490.1 helix-turn-helix domain-containing protein -
  LU301_RS07840 (LU301_07825) pilF 1577141..1577965 (-) 825 WP_305274023.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LU301_RS07845 (LU301_07830) rlmN 1577984..1579138 (-) 1155 Protein_1519 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  LU301_RS07850 (LU301_07835) ndk 1579335..1579766 (-) 432 WP_305269492.1 nucleoside-diphosphate kinase -
  LU301_RS07855 (LU301_07840) - 1580110..1582044 (+) 1935 WP_305269495.1 autotransporter outer membrane beta-barrel domain-containing protein -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 30822.32 Da        Isoelectric Point: 9.6637

>NTDB_id=639627 LU301_RS07840 WP_305274023.1 1577141..1577965(-) (pilF) [Moraxella sp. ZY210820]
MQQKWLSFMLIATISSIGLVGCETTTVIGAPVQKEVKKDPERAIQTRTQLAAEYIRSGDLDAAKRSLDQALQINSRSAEA
NMMMGVLLQTEGSPTNIAKAEQYYRTAVSADPKNARIRLNYGTYLFMIKKYAAAIENLKIAGTTLGYEQRAVALENLGMV
YLALNDLNNAEQAFKQSIQADRSGSPAAYFELAEIFYLKKDFNSAAAAYEQFSRRVGKEQLNSRALWIELRIARAFNDKT
KVQILANQMRALYPDSVEYQRYLKLKDSSEVVWK

Nucleotide


Download         Length: 825 bp        

>NTDB_id=639627 LU301_RS07840 WP_305274023.1 1577141..1577965(-) (pilF) [Moraxella sp. ZY210820]
ATGCAACAAAAGTGGTTGAGTTTTATGCTTATTGCTACAATAAGTTCAATTGGACTGGTTGGCTGTGAAACAACAACAGT
AATAGGTGCACCTGTACAAAAGGAAGTGAAGAAAGACCCTGAACGTGCAATCCAAACACGCACACAATTGGCTGCTGAAT
ATATCCGTTCTGGTGATTTAGATGCGGCAAAACGCTCACTTGATCAGGCATTACAAATTAACTCACGCAGTGCTGAAGCA
AATATGATGATGGGGGTATTGTTGCAAACAGAGGGTAGTCCAACAAATATCGCCAAAGCTGAACAATATTATCGTACTGC
GGTAAGTGCTGACCCTAAAAATGCACGCATTCGTCTTAATTATGGTACATATTTATTTATGATTAAAAAATATGCCGCTG
CGATTGAAAATTTAAAAATTGCGGGAACAACATTGGGTTATGAACAACGTGCGGTAGCTTTAGAAAATTTAGGTATGGTA
TATTTGGCATTAAATGATTTAAATAATGCCGAGCAAGCATTTAAGCAATCGATACAAGCAGACCGTTCGGGTTCTCCTGC
TGCATATTTTGAGTTGGCTGAAATTTTTTATTTAAAGAAAGATTTTAACAGTGCGGCAGCTGCTTATGAGCAATTTTCAC
GCCGTGTAGGTAAGGAACAATTAAATTCTCGTGCATTGTGGATTGAGTTGCGTATTGCACGTGCTTTTAATGATAAAACC
AAAGTACAAATTTTAGCAAATCAAATGCGTGCATTATATCCTGATAGTGTCGAATATCAACGTTATTTAAAACTTAAAGA
TAGTTCAGAGGTCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(47-74)

Tetratricopeptide repeat (TPR_16)

(96-136)

Tetratricopeptide repeat (TPR_16)

(156-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

55.185

98.54

0.544
  pilF Acinetobacter baylyi ADP1

52.471

95.985

0.504