Detailed information NTG0639510

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: LDE72_RS01750
• Genome accession: NZ_CP089962
• Coordinates: 393697..395070 (+)
• Length: 457 a.a.
• NCBI ID: WP_078009726.1
• Uniprot ID: A0A1S9AN20
• Organism: Limosilactobacillus reuteri strain NL02
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 388697..400070

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LDE72_RS01720 (LDE72_01700)	-	388972..389721 (+)	750	WP_085719646.1	amino acid ABC transporter ATP-binding protein	-
LDE72_RS01725 (LDE72_01705)	-	389737..390528 (+)	792	WP_003670552.1	transporter substrate-binding domain-containing protein	-
LDE72_RS01730 (LDE72_01710)	-	390636..391976 (-)	1341	WP_003670551.1	aminopeptidase C	-
LDE72_RS01735 (LDE72_01715)	rpiA	392005..392688 (-)	684	WP_098035163.1	ribose-5-phosphate isomerase RpiA	-
LDE72_RS01740 (LDE72_01720)	-	392700..392996 (-)	297	WP_029507893.1	GNAT family N-acetyltransferase	-
LDE72_RS01745 (LDE72_01725)	-	393135..393671 (+)	537	WP_003667334.1	dUTP diphosphatase	-
LDE72_RS01750 (LDE72_01730)	radA	393697..395070 (+)	1374	WP_078009726.1	DNA repair protein RadA	Machinery gene
LDE72_RS01755 (LDE72_01735)	-	395088..396245 (+)	1158	WP_003670546.1	PIN/TRAM domain-containing protein	-
LDE72_RS01760 (LDE72_01740)	cysS	396626..398038 (+)	1413	WP_305068375.1	cysteine--tRNA ligase	-
LDE72_RS01765 (LDE72_01745)	-	398050..398463 (+)	414	WP_003670544.1	Mini-ribonuclease 3	-
LDE72_RS01770 (LDE72_01750)	rlmB	398453..399202 (+)	750	WP_003667343.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
LDE72_RS01775 (LDE72_01755)	rpmG	399268..399417 (+)	150	WP_003666293.1	50S ribosomal protein L33	-
LDE72_RS01780 (LDE72_01760)	secE	399429..399605 (+)	177	WP_003666294.1	preprotein translocase subunit SecE	-

Sequence

Protein

• Length: 457 a.a.
• Molecular weight: 49963.27 Da
• Isoelectric Point: 8.4221

>NTDB_id=639510 LDE72_RS01750 WP_078009726.1 393697..395070(+) (radA) [Limosilactobacillus reuteri strain NL02]
MAKIRTQYVCQNCGYNSPRYLGRCPNCGEWNTLVEEQVETSSAPTKKATTTLTGLVAKPQKINEIDSTETPRVKTKLNEL
NRVLGGGIVPGSLILIGGDPGIGKSTLLLQVSGQLSDEHHRVLYVSGEESGTQIKMRAERLKVAGDDFYVYPETNMDSIR
DTIRDLKPEYVVIDSVQTMQATDVSSAIGSVSQIREVTAQLMQIAKSNNITIFVVGHVTKGGAIAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMHTNGLTEVKNPSEIFLEERLHDATGSAVVVSLEGTRPILVEIQALVTPTVY
GNAQRTATGLSRNRVSLIMAVLEKRANLMLQNQDAYLKAAGGVKLDEPAIDLAIAVSIASSYRDKGTKPTDAFVGEVGLT
GEIRRVNRIEQRVAEAEKLGFKRIFIPKNNLKGWKPSTNIQVVGVSTLKEALYLALG

Nucleotide

• Length: 1374 bp

>NTDB_id=639510 LDE72_RS01750 WP_078009726.1 393697..395070(+) (radA) [Limosilactobacillus reuteri strain NL02]
ATGGCAAAAATTCGAACACAATATGTTTGCCAAAACTGTGGTTACAACTCACCACGCTACTTAGGCCGGTGTCCAAATTG
TGGTGAGTGGAATACTTTAGTGGAAGAACAGGTAGAGACCAGTTCTGCACCAACCAAAAAAGCAACTACCACTTTAACCG
GCTTAGTTGCCAAACCACAGAAAATTAATGAGATTGATAGTACAGAGACTCCCCGTGTTAAAACAAAATTGAATGAGTTA
AATCGCGTTTTAGGTGGAGGAATCGTTCCCGGATCACTAATTCTAATTGGAGGAGATCCGGGAATCGGAAAATCGACCCT
CCTTTTACAAGTTTCCGGACAATTAAGTGATGAGCATCACCGGGTTTTATATGTGTCTGGAGAAGAAAGTGGGACACAAA
TTAAAATGCGGGCGGAACGATTAAAAGTTGCTGGGGATGACTTTTATGTATATCCAGAAACGAATATGGATAGTATTCGG
GATACTATTCGTGACCTCAAGCCAGAGTATGTCGTGATTGACTCGGTACAAACGATGCAGGCGACTGATGTAAGTTCAGC
AATTGGTAGTGTATCGCAAATTCGTGAAGTTACCGCCCAGTTAATGCAAATTGCTAAAAGTAATAACATTACGATTTTTG
TCGTTGGACATGTGACAAAGGGCGGTGCAATCGCTGGTCCAAAGATCTTGGAGCACATGGTTGATACAGTCTTGTATTTT
GAAGGAGACCTGCACCATACATATCGTATTTTGCGATCAGTGAAAAATCGATTTGGTTCAACTAACGAGCTTGGTATTTT
TGAAATGCATACAAATGGGCTGACGGAAGTAAAAAATCCATCAGAAATCTTTTTAGAGGAACGATTACATGATGCAACCG
GTTCAGCGGTGGTTGTTTCATTAGAAGGAACACGACCAATTTTAGTTGAAATTCAAGCGCTAGTAACACCGACCGTTTAT
GGAAATGCACAGCGAACTGCTACCGGTTTGAGCCGAAACCGAGTATCATTGATAATGGCGGTATTAGAGAAACGTGCTAA
CCTAATGCTTCAAAATCAAGATGCTTACTTAAAAGCAGCTGGGGGCGTTAAGTTAGATGAGCCAGCAATTGATTTGGCAA
TTGCAGTAAGTATTGCCTCCAGTTATCGGGATAAAGGAACCAAGCCCACCGATGCATTTGTCGGTGAAGTAGGTTTGACT
GGGGAAATTCGCCGGGTAAACCGAATTGAACAGCGGGTTGCTGAAGCAGAAAAACTTGGCTTTAAGCGAATCTTTATTCC
CAAAAATAATTTAAAGGGTTGGAAACCGTCAACTAATATTCAAGTAGTCGGTGTCTCAACGCTTAAAGAGGCGTTATATT
TAGCGCTGGGGTAA

Domains

Predicted by InterproScan.

• Subunit ChlI of Mg-chelatase (ChlI) (363-430)

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

• AAA domain (AAA_25) (65-222)

3D structure

• AlphaFold DB: A0A1S9AN20

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Streptococcus mitis NCTC 12261	66.372	98.906	0.656
radA	Streptococcus mitis SK321	66.372	98.906	0.656
radA	Streptococcus pneumoniae Rx1	66.15	98.906	0.654
radA	Streptococcus pneumoniae D39	66.15	98.906	0.654
radA	Streptococcus pneumoniae R6	66.15	98.906	0.654
radA	Streptococcus pneumoniae TIGR4	66.15	98.906	0.654
radA	Bacillus subtilis subsp. subtilis str. 168	62.801	100	0.628