Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GY50_RS00270 Genome accession   NC_022964
Coordinates   53416..54804 (+) Length   462 a.a.
NCBI ID   WP_023651723.1    Uniprot ID   -
Organism   Dehalococcoides mccartyi GY50     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 48416..59804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GY50_RS00260 (GY50_0056) - 50367..52841 (+) 2475 WP_023651721.1 ATP-dependent Clp protease ATP-binding subunit -
  GY50_RS00265 (GY50_0057) - 52964..53353 (+) 390 WP_023651722.1 hypothetical protein -
  GY50_RS00270 (GY50_0058) radA 53416..54804 (+) 1389 WP_023651723.1 DNA repair protein RadA Machinery gene
  GY50_RS00275 (GY50_0059) - 54791..55474 (+) 684 WP_023651724.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GY50_RS00280 (GY50_0060) ispF 55480..55953 (+) 474 WP_023651725.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  GY50_RS00285 (GY50_0061) cysS 55957..57330 (+) 1374 WP_023651726.1 cysteine--tRNA ligase -
  GY50_RS07445 (GY50_0062) - 58005..59054 (-) 1050 Protein_57 recombinase family protein -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 49556.95 Da        Isoelectric Point: 7.2840

>NTDB_id=63856 GY50_RS00270 WP_023651723.1 53416..54804(+) (radA) [Dehalococcoides mccartyi GY50]
MEKSRRVYVCSNCGRESLKWLGRCPGCQEWNTLEETMVVSPVGRKNEFARVISPAAELSSLQAAETTRRSLSISEFNRVL
GGGIVPGSLMLLGGEPGIGKSTLLLQVAASVAQSGGKVVYVSGEENPAQIKMRAQRLGISGEGLFLMAETDLNAILAQLS
VLSPSLVVIDSIQTVFLPELEAAPGAINQVRESALRLMQWAKSSGASVFITAHVTKEGNIAGPRILEHIVDVVMYFEGES
QSAYRLVRSVKNRFGSTNEVGIFEMKSEGLVEVANPSQIFLSNRQTNTVGSAVTAVLEGSRPLLVEVQALTNTTSFGQPR
RTANGVDFNRTLMIAAVLSKRLSMRLGTQDIIVNATGGIRLDEPAADLAIALAIASSYRDIGVCPETIALGEIGLSGEMR
TISHLERRLSEASRLGFKRALVPAGANCHNVHNIQIIPVSTVKEAVRLALTGVKTETEDVFE

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=63856 GY50_RS00270 WP_023651723.1 53416..54804(+) (radA) [Dehalococcoides mccartyi GY50]
ATGGAAAAAAGCCGTAGAGTTTATGTTTGTTCCAACTGCGGGCGTGAGAGCCTGAAGTGGCTGGGGCGCTGTCCCGGCTG
TCAGGAATGGAATACCCTTGAAGAAACAATGGTTGTTTCACCTGTAGGGCGTAAAAATGAATTTGCCCGTGTTATCAGCC
CGGCGGCTGAGCTTTCCAGTCTGCAGGCAGCCGAAACCACCCGGCGGAGTTTATCTATTTCGGAGTTCAACCGGGTATTA
GGCGGCGGCATAGTGCCGGGTTCGCTTATGCTTTTGGGTGGTGAGCCGGGAATCGGCAAATCCACTCTTCTTCTTCAGGT
GGCGGCTTCGGTAGCACAAAGCGGCGGTAAGGTAGTATATGTTTCAGGGGAAGAAAACCCCGCTCAGATAAAAATGCGCG
CCCAACGTCTTGGTATCAGCGGCGAAGGGCTTTTCCTTATGGCTGAGACCGACCTTAATGCCATTCTGGCTCAGCTTTCA
GTCCTCAGCCCGTCATTGGTCGTTATTGACTCTATCCAAACTGTATTCCTGCCGGAGCTTGAGGCCGCACCCGGTGCTAT
AAATCAGGTGCGTGAATCAGCCCTCCGCCTTATGCAGTGGGCTAAAAGCAGCGGTGCCAGTGTATTTATAACAGCCCATG
TTACTAAAGAAGGCAATATAGCCGGGCCGCGCATATTGGAACACATAGTAGATGTAGTCATGTACTTTGAGGGAGAGTCC
CAGAGTGCTTACCGTTTAGTGCGTTCTGTCAAAAACCGCTTCGGTTCCACTAACGAAGTGGGTATATTTGAAATGAAAAG
TGAAGGTCTGGTAGAAGTAGCTAACCCTTCCCAGATATTTTTATCAAATCGCCAGACAAACACGGTAGGCTCGGCTGTAA
CCGCTGTACTGGAGGGCAGCCGTCCTTTGCTGGTTGAGGTGCAGGCACTTACCAATACCACCAGTTTCGGCCAGCCGCGC
CGCACTGCCAACGGGGTGGATTTTAACCGCACCCTTATGATAGCTGCGGTCCTCTCCAAACGCCTGTCCATGCGTTTAGG
TACGCAGGACATTATTGTAAACGCCACCGGCGGTATCCGTTTGGATGAGCCGGCTGCAGATTTGGCTATTGCACTGGCTA
TTGCTTCCAGTTACCGGGATATCGGCGTATGCCCCGAAACTATCGCTCTGGGAGAGATTGGTCTTTCTGGGGAAATGCGG
ACTATTTCCCATCTGGAAAGGCGTCTTTCCGAGGCCAGCCGTTTGGGTTTTAAAAGGGCTTTGGTACCGGCCGGTGCTAA
CTGCCACAATGTACATAATATCCAGATAATTCCGGTTTCAACTGTCAAAGAGGCAGTGAGGCTGGCACTTACCGGGGTGA
AAACGGAGACGGAAGATGTTTTTGAATGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(342-424)

AAA domain (AAA_25)

(63-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.327

99.351

0.5
  radA Streptococcus pneumoniae Rx1

49.119

98.268

0.483
  radA Streptococcus pneumoniae D39

49.119

98.268

0.483
  radA Streptococcus pneumoniae R6

49.119

98.268

0.483
  radA Streptococcus pneumoniae TIGR4

49.119

98.268

0.483
  radA Streptococcus mitis SK321

49.119

98.268

0.483
  radA Streptococcus mitis NCTC 12261

48.899

98.268

0.481

Multiple sequence alignment