Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   T285_RS08005 Genome accession   NC_022909
Coordinates   1700845..1702653 (+) Length   602 a.a.
NCBI ID   WP_023599983.1    Uniprot ID   A0A7D9N8B8
Organism   Lactobacillus johnsonii N6.2     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1695845..1707653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  T285_RS07980 (T285_08255) - 1696225..1697007 (-) 783 WP_023599978.1 threonine/serine exporter family protein -
  T285_RS07985 (T285_08260) - 1697024..1698571 (-) 1548 WP_023599979.1 ABC-F family ATP-binding cassette domain-containing protein -
  T285_RS07990 (T285_08265) - 1698571..1698954 (-) 384 WP_023599980.1 hypothetical protein -
  T285_RS07995 (T285_08270) - 1699180..1699452 (+) 273 WP_023599981.1 hypothetical protein -
  T285_RS08000 (T285_08275) - 1699557..1700681 (-) 1125 WP_023599982.1 LCP family protein -
  T285_RS08005 (T285_08280) pepF 1700845..1702653 (+) 1809 WP_023599983.1 oligoendopeptidase F Regulator
  T285_RS08010 (T285_08285) - 1702723..1704234 (-) 1512 WP_023599984.1 lactate permease LctP family transporter -
  T285_RS08015 (T285_08290) - 1704355..1705146 (-) 792 WP_023599985.1 metal ABC transporter permease -
  T285_RS08020 (T285_08295) - 1705146..1705805 (-) 660 WP_023599986.1 ATP-binding cassette domain-containing protein -
  T285_RS08025 (T285_08300) - 1705842..1706741 (-) 900 WP_011162512.1 zinc ABC transporter substrate-binding protein -
  T285_RS08030 (T285_08305) - 1707043..1707321 (-) 279 WP_011162513.1 metalloregulator ArsR/SmtB family transcription factor -

Sequence

Protein


Download         Length: 602 a.a.        Molecular weight: 68651.43 Da        Isoelectric Point: 4.6397

>NTDB_id=63833 T285_RS08005 WP_023599983.1 1700845..1702653(+) (pepF) [Lactobacillus johnsonii N6.2]
MALPKRNEVSDDLKWDLSRIFKDDHEWEQEYNQLTTEIKNLGRFKGNLAKSGQDLYEGITEILSVNRRLEKVYVYATMSS
DVDTSNTHYLGLVAKAQSLANQMSAAISFVDPEILSIPEETLAKFMQDEPRLKEYQHRLEQITKKRPHTLPASEEKIIAA
AGDAMGTSANTFNVLTNSDMEYGYVQDEDGEMVQLSDGLYSLLIQSQDRNIRKNAFDVMYASYGQFENSLASTLSGEVKA
HNFNAQVHKYNSAREAALSENGVPTAVYDTLVKEVNSHLNLLHRYVSLRKKILGLSDLQMYDMYVPLTGEPSLSYNFEEA
KNEARQALAPLGKDYLEHVDYIFNNRVIDVVESQNKVTGAYSGGSYDTDPYELLNWEDNLDSLYTLVHETGHSVHSWYTR
NTQPYVYGDYPIFVAEIASTTNENILTEYFLDKITDPKTRAFVLNHYLDSFKGTLFRQTQFAEFEQFIHEADANGQPLTA
DVLDEFYGNLNQRYYGDSVEPGGEIAKEWARIPHFYYNFYVYQYATGFAAATALANNVVHGTDKERTAYINFLKAGSSDY
PTEIMKCAGVDMTKADYLRDAFNTFEKRLNEFEKIVNELKTI

Nucleotide


Download         Length: 1809 bp        

>NTDB_id=63833 T285_RS08005 WP_023599983.1 1700845..1702653(+) (pepF) [Lactobacillus johnsonii N6.2]
ATGGCTCTTCCAAAAAGAAATGAAGTATCTGATGATCTCAAGTGGGATCTTAGTCGTATTTTTAAAGACGATCACGAATG
GGAACAAGAATATAATCAGCTCACAACAGAAATCAAAAATTTAGGTAGATTTAAGGGAAATCTTGCTAAATCTGGTCAAG
ATTTATATGAGGGGATTACAGAAATTTTATCTGTGAATCGTCGTTTAGAAAAAGTATATGTTTATGCTACTATGTCAAGC
GACGTTGATACTAGTAATACTCATTATCTAGGCCTTGTTGCTAAAGCTCAAAGCTTAGCTAACCAGATGAGCGCCGCTAT
CTCTTTTGTCGATCCTGAAATTTTATCTATCCCAGAAGAGACACTTGCTAAATTTATGCAAGATGAGCCCCGTTTAAAAG
AGTATCAACATCGACTTGAACAAATTACTAAGAAACGTCCTCATACTTTACCAGCAAGTGAAGAAAAAATTATTGCCGCT
GCTGGAGATGCAATGGGAACTTCCGCTAATACTTTCAATGTTTTAACTAATTCTGATATGGAATACGGCTATGTTCAAGA
TGAAGACGGAGAGATGGTCCAATTATCTGATGGCTTATATTCACTTCTAATTCAGTCACAAGATAGAAATATTCGCAAAA
ATGCATTTGATGTTATGTATGCAAGCTATGGCCAGTTTGAAAATAGTCTCGCTTCAACTTTATCAGGTGAAGTAAAAGCC
CACAATTTTAATGCTCAAGTACATAAATATAATTCAGCTCGAGAGGCTGCTTTAAGTGAAAATGGCGTACCAACAGCTGT
TTACGATACATTGGTTAAAGAGGTAAATAGTCACCTCAACTTACTTCATCGCTACGTTAGTTTAAGAAAAAAGATTTTAG
GTCTTTCTGATTTACAAATGTATGATATGTATGTTCCATTAACAGGTGAGCCCAGTCTTTCTTATAACTTTGAAGAAGCA
AAAAATGAGGCTAGACAAGCCTTAGCGCCATTAGGAAAAGATTACCTCGAACATGTTGATTACATCTTTAATAATCGTGT
AATTGATGTGGTTGAAAGCCAAAATAAGGTAACAGGCGCTTATTCTGGTGGTTCCTATGATACTGATCCATATGAATTAT
TAAATTGGGAGGATAATCTTGATTCTCTATATACTCTAGTTCATGAAACTGGACACTCAGTGCATTCCTGGTATACAAGA
AATACTCAACCATATGTATATGGAGACTACCCAATTTTTGTTGCTGAAATTGCATCAACAACTAATGAAAATATTTTAAC
TGAATACTTCTTAGATAAAATTACCGATCCTAAAACACGCGCATTTGTTTTGAACCACTATCTTGATTCATTCAAGGGCA
CACTCTTCCGTCAAACACAATTTGCAGAATTTGAGCAGTTTATTCATGAAGCTGACGCAAATGGTCAACCATTAACTGCC
GATGTTCTTGATGAATTTTACGGCAATTTAAACCAAAGATATTACGGTGATAGTGTAGAACCGGGCGGAGAGATTGCTAA
AGAATGGGCCCGTATTCCTCACTTCTATTACAACTTTTATGTTTACCAATATGCAACAGGCTTTGCTGCTGCTACAGCTC
TCGCAAATAATGTTGTTCATGGTACTGACAAAGAACGTACCGCTTATATTAATTTCTTAAAGGCTGGCTCTTCAGATTAT
CCAACAGAGATAATGAAATGTGCCGGTGTCGATATGACAAAGGCTGATTACTTAAGAGACGCATTTAATACTTTTGAAAA
ACGACTTAATGAGTTTGAAAAAATCGTTAATGAACTGAAAACAATCTAG

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(113-181)

Peptidase family M3 (Peptidase_M3)

(203-583)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D9N8B8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

51.858

98.339

0.51

Multiple sequence alignment