Detailed information NTG0638328

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: LRS65_RS25460
• Genome accession: NZ_CP089601
• Coordinates: 4954370..4955719 (-)
• Length: 449 a.a.
• NCBI ID: WP_257115774.1
• Uniprot ID: -
• Organism: Bacillus cereus strain C-1
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4949370..4960719

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LRS65_RS25440 (LRS65_25400)	secA	4949695..4952202 (-)	2508	WP_000579385.1	preprotein translocase subunit SecA	-
LRS65_RS25445 (LRS65_25405)	raiA	4952477..4953019 (-)	543	WP_000671185.1	ribosome-associated translation inhibitor RaiA	-
LRS65_RS25450 (LRS65_25410)	cspC	4953342..4953539 (-)	198	WP_001990088.1	cold shock protein CspC	-
LRS65_RS25455 (LRS65_25415)	-	4953666..4954370 (-)	705	WP_013142274.1	ComF family protein	-
LRS65_RS25460 (LRS65_25420)	comFA	4954370..4955719 (-)	1350	WP_257115774.1	ATP-dependent helicase ComFA	Machinery gene
LRS65_RS25465 (LRS65_25425)	-	4955847..4957268 (-)	1422	WP_107992274.1	C40 family peptidase	-
LRS65_RS25470 (LRS65_25430)	-	4957417..4957731 (-)	315	WP_000400857.1	helix-turn-helix domain-containing protein	-
LRS65_RS25475 (LRS65_25435)	-	4957904..4958746 (-)	843	WP_025921785.1	DegV family protein	-
LRS65_RS25480 (LRS65_25440)	-	4958982..4959617 (+)	636	WP_063538078.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51083.96 Da
• Isoelectric Point: 9.9041

>NTDB_id=638328 LRS65_RS25460 WP_257115774.1 4954370..4955719(-) (comFA) [Bacillus cereus strain C-1]
MVAGKQLLLEEISSGLQRELNDLKKKGEVICVQGVKKKNSKYMCQRCGNVDRRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIAERKREKNLNLLQWNGTLSAGQDLAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVVALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVIVVDEIDAFPYCADQMLQYAVKQAM
KEKAARIYLTATPDETWKRKFRKGEQKGVIVSGRYHRHPLPVPLFCWCGNWKKSLNRERIPRVLLQWLKMYLNKKYPVFL
FVPHVRYIEEISSLLKSLHNKVEGVHAEDPMRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=638328 LRS65_RS25460 WP_257115774.1 4954370..4955719(-) (comFA) [Bacillus cereus strain C-1]
ATGGTAGCGGGGAAACAGTTGCTACTAGAAGAAATTTCTTCAGGTTTACAGAGGGAATTAAATGATTTGAAAAAGAAGGG
AGAGGTCATATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGATGTGGAAATGTAGATCGGCGGC
TATTTGCGTCGTTTTTATGTAAAAGGTGCAGTAAAGTGTGCACATATTGCCGGAAGTGTATAACGATGGGGAGGGTAAGT
GAATGTGCTGTACTTGTTCGAGGGATTGCTGAAAGAAAGAGAGAAAAGAATTTAAACTTGTTACAGTGGAACGGGACGTT
GTCTGCTGGACAAGATTTGGCGGCGCAAGGAGTTATAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGTATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCAAGAACGGATGTTGTTTTGGAATTAGCACCGAGATTACAAGAAGTATTTCCATATATAAAGGTAGTGGCTTTATATGG
AGGGAGTGTAGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACCACGCATCAATTATTACGTTATTATAGGGCGTTTC
ATGTCATAGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAAAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAATTTAGAAAAGGTGAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCGTTGCCAGTTCCTCTATTTTGCTGGTGCGGAAATTGGAAGAAAA
GCCTCAACCGTGAAAGAATTCCTCGAGTTTTACTACAATGGTTAAAGATGTACTTAAACAAAAAGTATCCTGTTTTTTTA
TTCGTTCCCCATGTACGATATATAGAAGAAATAAGCTCGTTATTGAAATCATTGCATAATAAAGTTGAAGGGGTACATGC
AGAAGATCCGATGAGAAAAGAGAAAGTCGCAGCGTTCAGAAAGGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTTGCAGTTTTAGGGGCGGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCATATGGAGAGGTCATTTATTTTCACTATGGCAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (313-408)

• Type III restriction enzyme, res subunit (ResIII) (107-254)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	52.153	93.096	0.486