Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   T285_RS01825 Genome accession   NC_022909
Coordinates   409717..411096 (+) Length   459 a.a.
NCBI ID   WP_023599241.1    Uniprot ID   -
Organism   Lactobacillus johnsonii N6.2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 404717..416096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  T285_RS01805 (T285_01870) - 405169..406182 (-) 1014 WP_041818441.1 sugar transferase -
  T285_RS01810 (T285_01875) pepC 406467..407816 (-) 1350 WP_023599239.1 aminopeptidase C -
  T285_RS01815 (T285_01880) - 408013..409047 (+) 1035 WP_023599240.1 LCP family protein -
  T285_RS01820 (T285_01885) - 409166..409717 (+) 552 WP_004896724.1 dUTP diphosphatase -
  T285_RS01825 (T285_01890) radA 409717..411096 (+) 1380 WP_023599241.1 DNA repair protein RadA Machinery gene
  T285_RS01830 (T285_01895) gltX 411178..412677 (+) 1500 WP_023599242.1 glutamate--tRNA ligase -
  T285_RS01835 (T285_01900) cysS 412767..414179 (+) 1413 WP_023599243.1 cysteine--tRNA ligase -
  T285_RS01840 (T285_01905) - 414172..414615 (+) 444 WP_004895772.1 ribonuclease III domain-containing protein -
  T285_RS01845 (T285_01910) rlmB 414602..415357 (+) 756 WP_004895769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50134.70 Da        Isoelectric Point: 7.6366

>NTDB_id=63822 T285_RS01825 WP_023599241.1 409717..411096(+) (radA) [Lactobacillus johnsonii N6.2]
MAKVKTRYKCRNCGYISASYLGRCPNCGAWNQFEEETQEIKKVSTKATASRLMTKIGNNDPVKLTEVKAEKEKRIVTPFE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQITGALAKEHSVLYVSGEESASQIKMRADRLGVSNSGILLYPETNMQNI
RDQIDEIKPDFLVIDSIQTMNEPSLDSMVGSASQVREVTSELMKIAKNEQITTFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDGHHSYRILRSVKNRFGAANEIGMFEMKNEGLAEVSNPSAIFLDERLPNSTGSSVVVSLEGTRPLLADIQALVTPTA
FGYAKRTTSGLDFNRVALLLSVLEKRGNLMLQNQDAFLTATGGIKLNEPAIDLAICMAVASSYKNKEISSTDCFVGEVGL
TGEIRRVNQIEARVKEAAKVGFKRIFIPKNNLNQELKNNSEIEVIGVASLPQALKLVFN

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=63822 T285_RS01825 WP_023599241.1 409717..411096(+) (radA) [Lactobacillus johnsonii N6.2]
ATGGCAAAAGTTAAAACTCGTTATAAGTGTCGTAATTGTGGATATATTTCTGCTTCCTATCTTGGAAGATGTCCCAATTG
TGGTGCTTGGAATCAATTTGAAGAAGAGACACAGGAAATTAAAAAAGTATCAACTAAGGCAACTGCAAGTCGGTTGATGA
CTAAAATTGGAAATAATGATCCAGTAAAGTTAACTGAAGTAAAAGCTGAGAAAGAAAAAAGAATTGTGACTCCTTTTGAA
GAATTAAATCGAGTTTTAGGAGGAGGGATTGTTCCAGGATCTTTAGTTTTAATTGGAGGAGATCCAGGAATTGGAAAATC
AACCTTAATGCTTCAAATTACTGGTGCTTTAGCTAAAGAACATAGCGTCTTATATGTTTCAGGAGAAGAGTCCGCAAGTC
AAATAAAGATGCGGGCAGATCGATTAGGAGTAAGCAATAGCGGAATTTTGCTTTATCCAGAAACAAATATGCAGAATATT
CGTGACCAGATTGATGAAATTAAGCCAGATTTTTTGGTAATTGATTCTATTCAAACAATGAATGAACCTTCACTTGATTC
AATGGTTGGATCTGCTTCTCAAGTACGTGAAGTAACAAGTGAACTAATGAAAATTGCTAAAAATGAACAAATAACTACTT
TTGTTATTGGCCATGTAACTAAAGAAGGAGCAATTGCTGGGCCTAAGATTATGGAACATATGGTTGATACCGTTCTTTAC
TTTGAAGGAGATGGGCACCACTCATATCGAATTTTAAGATCAGTAAAGAATCGTTTTGGTGCAGCTAATGAAATTGGAAT
GTTTGAGATGAAAAATGAAGGGTTAGCAGAAGTAAGTAATCCTTCAGCTATTTTCTTAGACGAACGCTTACCTAATTCGA
CTGGTTCCTCAGTTGTTGTTTCACTTGAAGGAACGCGGCCTCTTCTTGCAGATATCCAAGCTTTAGTAACCCCAACAGCA
TTCGGATATGCAAAAAGAACAACTTCAGGATTAGATTTTAATCGAGTAGCACTTTTATTGTCAGTTTTAGAAAAAAGAGG
CAACTTAATGCTTCAAAATCAAGATGCTTTTCTAACTGCAACTGGTGGGATTAAATTAAATGAACCAGCAATTGATCTTG
CAATTTGCATGGCAGTTGCTTCAAGTTATAAAAATAAAGAAATTTCTTCGACTGATTGCTTTGTAGGTGAAGTTGGCTTG
ACTGGTGAAATTAGAAGAGTTAATCAAATTGAAGCTAGAGTTAAAGAGGCCGCAAAAGTAGGCTTTAAGCGAATTTTTAT
TCCTAAAAACAACTTAAACCAGGAGCTTAAGAATAACTCTGAGATCGAAGTAATTGGAGTAGCAAGTTTACCACAAGCTT
TAAAACTTGTTTTTAACTAG

Domains

Predicted by InterProScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

60.044

99.782

0.599
  radA Streptococcus mitis SK321

60.044

99.782

0.599
  radA Streptococcus pneumoniae Rx1

60.044

99.782

0.599
  radA Streptococcus pneumoniae D39

60.044

99.782

0.599
  radA Streptococcus pneumoniae R6

60.044

99.782

0.599
  radA Streptococcus pneumoniae TIGR4

60.044

99.782

0.599
  radA Bacillus subtilis subsp. subtilis str. 168

55.921

99.346

0.556

Multiple sequence alignment