Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   U875_RS20445 Genome accession   NC_022904
Coordinates   4558892..4560379 (+) Length   495 a.a.
NCBI ID   WP_023597761.1    Uniprot ID   A0A378YTJ4
Organism   Pandoraea pnomenusa 3kgm     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4553892..4565379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U875_RS20420 (U875_20885) - 4555052..4556032 (+) 981 WP_023597756.1 KpsF/GutQ family sugar-phosphate isomerase -
  U875_RS20425 (U875_20890) - 4556140..4556625 (+) 486 WP_048806815.1 KdsC family phosphatase -
  U875_RS20430 lptC 4556650..4557309 (+) 660 WP_023597758.1 LPS export ABC transporter periplasmic protein LptC -
  U875_RS20435 (U875_20900) lptA 4557306..4558004 (+) 699 WP_023597759.1 lipopolysaccharide transport periplasmic protein LptA -
  U875_RS20440 (U875_20905) lptB 4558027..4558797 (+) 771 WP_028731000.1 LPS export ABC transporter ATP-binding protein -
  U875_RS20445 (U875_20910) rpoN/rpoN1 4558892..4560379 (+) 1488 WP_023597761.1 RNA polymerase factor sigma-54 Machinery gene
  U875_RS20450 (U875_20915) hpf 4560516..4560869 (+) 354 WP_023597762.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  U875_RS20455 (U875_20920) ptsN 4561106..4561561 (+) 456 WP_023597763.1 PTS IIA-like nitrogen regulatory protein PtsN -
  U875_RS20460 (U875_20925) hprK 4561661..4562626 (+) 966 WP_023597764.1 HPr(Ser) kinase/phosphatase -
  U875_RS20465 (U875_20930) - 4562780..4563106 (+) 327 WP_023597765.1 PsiF family protein -
  U875_RS20470 (U875_20935) rapZ 4563287..4564159 (+) 873 WP_023597766.1 RNase adapter RapZ -
  U875_RS20475 (U875_20940) mutY 4564193..4565362 (-) 1170 WP_023597767.1 A/G-specific adenine glycosylase -

Sequence

Protein


Download         Length: 495 a.a.        Molecular weight: 55367.29 Da        Isoelectric Point: 5.5027

>NTDB_id=63809 U875_RS20445 WP_023597761.1 4558892..4560379(+) (rpoN/rpoN1) [Pandoraea pnomenusa 3kgm]
MKPTLQLRTSQHLTLTPQLQQSIRLLQLSTLELQQEVEHALTQNPMLEREDDWLAGSVRVGTDGSLRNERSTSASSASEG
HESANGHDETRELDGQTRFDDSNQDSGSDWSLNDFARSGSASDDDDNARPQLHVDHPNLREHLLAQLRLTKAGPRDRALV
EFLIESLDEDGYLGTMLDEIQADMPEELALETEEINAALALLQSFDPAGVGARTPSECLRLQLQRLPPSNVRTLSLRIVS
DHLELLAARDYTRLRRLLGVSEDSLRAAHHLIRSLDPFPGAAYGSPQADYVVPDVLVRKQGSNWIAELNPEVMPRLRINE
MYARILRNNRNDPGTGNLQQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFTHGEIGMRPLVLREIADTLGLHESTI
SRVTTSKYMLTPFGTFELKYFFGSHVATEAGGAASSTAIRALIKQLIGAEDPQSPLSDSRIAELLAEQGFVVARRTVAKY
REAMRIPAVNLRKSL

Nucleotide


Download         Length: 1488 bp        

>NTDB_id=63809 U875_RS20445 WP_023597761.1 4558892..4560379(+) (rpoN/rpoN1) [Pandoraea pnomenusa 3kgm]
ATGAAACCGACTCTTCAACTCCGCACATCGCAGCACCTGACCCTCACGCCGCAGTTGCAGCAATCCATCCGGTTGTTGCA
ACTGTCGACGCTCGAACTTCAGCAGGAAGTCGAGCACGCCCTCACCCAGAACCCGATGCTCGAGCGCGAGGACGACTGGC
TCGCGGGCTCGGTACGTGTCGGCACGGACGGGTCGCTTCGCAACGAGCGCAGCACCTCGGCGAGCAGCGCCAGCGAGGGC
CATGAGTCCGCCAACGGCCATGACGAAACCCGCGAGCTCGACGGCCAGACCCGCTTCGACGACAGCAACCAGGACAGCGG
CAGCGATTGGAGTCTTAACGATTTCGCCCGTTCGGGCAGCGCCTCGGACGACGACGACAACGCGCGCCCGCAGTTGCACG
TCGATCACCCCAACCTTCGCGAACACCTGCTCGCGCAGCTGCGCCTGACCAAGGCGGGGCCGCGCGATCGCGCGCTTGTC
GAATTCCTGATCGAATCGCTCGACGAAGACGGCTACCTCGGCACCATGCTCGACGAAATCCAGGCGGATATGCCTGAAGA
GCTGGCGCTCGAGACCGAGGAGATCAACGCGGCGCTCGCGCTCCTGCAGAGCTTCGATCCGGCCGGTGTGGGTGCCCGTA
CGCCGTCCGAATGCCTGCGCCTTCAGTTGCAGCGCCTGCCGCCGAGCAATGTGCGCACGCTCTCGCTGCGCATCGTCTCG
GATCACCTGGAACTGCTCGCCGCGCGCGATTACACGCGTTTGCGTCGCCTGCTCGGCGTGAGCGAAGACAGCCTGCGCGC
GGCGCATCACCTGATTCGCTCCCTCGACCCTTTCCCCGGCGCGGCCTACGGCTCGCCGCAGGCCGATTACGTGGTGCCCG
ACGTGCTGGTGCGCAAGCAGGGCAGCAACTGGATCGCCGAACTCAACCCCGAGGTCATGCCGCGGCTGCGCATCAACGAG
ATGTACGCGCGAATTCTGCGAAACAACCGCAACGATCCGGGCACCGGCAATCTGCAGCAACAGCTCCAGGAAGCCCGCTG
GCTGATCAAGAACATCCAGCAACGGTTCGACACGATCCTGCGTGTCGCACAGGCGATCGTCGAGCGTCAGAAGAACTTCT
TCACGCACGGCGAAATCGGCATGCGGCCCTTGGTTTTACGCGAGATTGCTGATACGCTGGGTTTACATGAATCCACGATT
TCACGGGTGACAACCAGTAAGTACATGCTCACTCCATTCGGGACTTTCGAGCTCAAGTACTTCTTTGGTTCGCACGTGGC
CACGGAGGCGGGCGGCGCAGCGTCATCGACGGCCATCCGCGCACTCATCAAGCAACTCATAGGAGCAGAAGACCCGCAGA
GCCCCCTTTCCGACAGCCGTATCGCGGAACTGCTGGCAGAGCAAGGGTTCGTGGTGGCGCGGCGCACTGTCGCGAAGTAC
CGCGAGGCCATGCGAATCCCCGCAGTCAACTTGCGCAAATCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(133-322)

Sigma-54, DNA binding domain (Sigma54_DBD)

(338-493)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378YTJ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.463

100

0.693

Multiple sequence alignment