Detailed information NTG0637903

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: LUS72_RS25705
• Genome accession: NZ_CP089518
• Coordinates: 4905061..4906410 (-)
• Length: 449 a.a.
• NCBI ID: WP_097832455.1
• Uniprot ID: A0A2B8T4B6
• Organism: Bacillus cereus strain PT1
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4900061..4911410

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LUS72_RS25680	-	4901601..4902539 (-)	939	WP_264448420.1	helix-turn-helix transcriptional regulator	-
LUS72_RS25685	-	4902622..4903065 (+)	444	WP_097832370.1	VOC family protein	-
LUS72_RS25690	hpf	4903171..4903713 (-)	543	WP_000671183.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
LUS72_RS25695	cspC	4904033..4904230 (-)	198	WP_001990088.1	cold shock protein CspC	-
LUS72_RS25700	-	4904357..4905061 (-)	705	WP_097832371.1	ComF family protein	-
LUS72_RS25705	comFA	4905061..4906410 (-)	1350	WP_097832455.1	ATP-dependent helicase ComFA	Machinery gene
LUS72_RS25710	-	4906541..4907884 (-)	1344	WP_264448421.1	NlpC/P60 family protein	-
LUS72_RS25715	-	4908117..4908431 (-)	315	WP_002112882.1	winged helix-turn-helix transcriptional regulator	-
LUS72_RS25720	-	4908604..4909446 (-)	843	WP_097832372.1	DegV family protein	-
LUS72_RS25725	-	4909685..4910320 (+)	636	WP_264448422.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 50904.36 Da
• Isoelectric Point: 9.6829

>NTDB_id=637903 LUS72_RS25705 WP_097832455.1 4905061..4906410(-) (comFA) [Bacillus cereus strain PT1]
MLAGRQLLLEELSSDLQNKLDHLKRNREVVCVQGVIKKFSKYTCQRCGNIEQRLFASFVCKRCSKVCAYCRKCITMGRVS
ECTVLVRGIAEISGENYLNPLQWEGVLSDGQELAAQSVVDAVKQKESFFIWAVCGAGKTEMLFRGIAEALQKGERVCIAT
PRTDVVLELAPRLQEVFPNINVAALYGGSVDREKGAALIVATTHQLLRYYRAFHVMIVDEIDAFPYHADKMLHYAVNKAM
KEEAARIYLTATPDEKWKRKLRNGKQKGVIISGRYHRHPLPVPLFRWCGNWKKGLMRKKIPRALLQWLNMYLSKQYPIFL
FVPHVRYVEEISQLLKSLNNQIDGVHAEDLARKEKVAAFRKGEIPLLITTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSSDEPHGDVIYFHYGKTEAMVRAKKHIQSMNKNAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=637903 LUS72_RS25705 WP_097832455.1 4905061..4906410(-) (comFA) [Bacillus cereus strain PT1]
ATGTTAGCTGGTAGACAGTTATTGTTAGAAGAACTTTCTTCAGATTTGCAGAATAAATTGGATCATTTGAAAAGGAACCG
AGAAGTCGTTTGTGTGCAAGGTGTAATTAAGAAATTTTCAAAATATACGTGCCAGCGTTGCGGAAATATAGAGCAACGAC
TATTTGCATCATTTGTATGTAAAAGGTGCAGTAAAGTATGTGCGTATTGTCGTAAGTGCATAACGATGGGCAGGGTAAGT
GAATGTACTGTACTTGTTCGCGGTATTGCTGAAATAAGCGGAGAAAATTATTTGAATCCGTTACAGTGGGAAGGCGTTTT
GTCTGATGGTCAGGAGTTAGCTGCGCAAAGTGTCGTTGACGCTGTTAAGCAGAAAGAATCATTTTTTATTTGGGCGGTGT
GCGGGGCTGGAAAAACAGAAATGTTGTTTCGCGGAATTGCAGAGGCACTACAAAAGGGAGAGAGAGTTTGTATTGCAACA
CCGAGAACGGACGTTGTACTTGAATTAGCACCAAGATTACAAGAAGTGTTTCCAAATATAAATGTAGCAGCTTTATACGG
AGGGAGTGTGGACCGAGAAAAGGGTGCGGCGTTAATCGTTGCAACTACACATCAATTGTTACGTTATTATAGAGCATTCC
ATGTCATGATTGTAGATGAGATAGATGCCTTTCCGTATCATGCGGATAAGATGTTGCATTATGCAGTAAACAAAGCGATG
AAAGAGGAGGCAGCACGTATTTATTTAACTGCAACTCCAGATGAAAAGTGGAAGCGTAAATTGCGGAATGGTAAGCAAAA
AGGGGTTATTATTTCAGGACGTTATCATCGTCATCCATTGCCAGTTCCACTATTTCGGTGGTGCGGAAATTGGAAAAAGG
GTCTTATGCGTAAAAAAATTCCTCGTGCTTTATTACAATGGTTAAATATGTACTTATCTAAACAATATCCCATTTTTCTA
TTTGTTCCTCATGTACGATATGTAGAGGAAATTAGCCAGTTATTAAAATCATTAAACAACCAAATTGATGGTGTGCATGC
AGAAGATCTGGCGAGAAAAGAGAAGGTAGCAGCTTTCAGAAAGGGAGAAATCCCTTTATTAATTACAACGACGATTTTGG
AACGAGGAGTAACTGTGAAAAATTTACAAGTGGCAGTTTTAGGGGCTGAGGAAGAAATATTTTCAGAAAGTGCTCTCGTT
CAAATTGCAGGGCGTGCTGGTAGAAGTTCTGATGAACCGCATGGAGATGTCATCTATTTTCATTATGGAAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAAGTATGAATAAAAATGCTAAAGAACAAGGGTTGATAGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (107-254)

• Helicase conserved C-terminal domain (Helicase_C) (313-409)

3D structure

• AlphaFold DB: A0A2B8T4B6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.02	98.218	0.501
comFA	Latilactobacillus sakei subsp. sakei 23K	40.955	88.641	0.363