Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LUS72_RS21015 Genome accession   NZ_CP089518
Coordinates   4044346..4045389 (-) Length   347 a.a.
NCBI ID   WP_097830696.1    Uniprot ID   -
Organism   Bacillus cereus strain PT1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4039346..4050389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LUS72_RS20970 - 4039747..4039947 (-) 201 WP_000106081.1 YqzE family protein -
  LUS72_RS20975 - 4039986..4040483 (-) 498 WP_097830703.1 shikimate kinase -
  LUS72_RS20980 - 4040634..4041284 (-) 651 WP_097830702.1 2OG-Fe(II) oxygenase -
  LUS72_RS20985 comGG 4041457..4041828 (-) 372 WP_097830701.1 competence type IV pilus minor pilin ComGG -
  LUS72_RS20990 comGF 4041825..4042286 (-) 462 WP_097830700.1 competence type IV pilus minor pilin ComGF -
  LUS72_RS20995 - 4042270..4042566 (-) 297 WP_097830699.1 competence protein ComG -
  LUS72_RS21000 comGD 4042559..4043014 (-) 456 WP_097830698.1 competence type IV pilus minor pilin ComGD -
  LUS72_RS21005 comGC 4043011..4043310 (-) 300 WP_002145747.1 competence type IV pilus major pilin ComGC -
  LUS72_RS21010 comGB 4043322..4044359 (-) 1038 WP_141533530.1 competence type IV pilus assembly protein ComGB -
  LUS72_RS21015 comGA 4044346..4045389 (-) 1044 WP_097830696.1 competence type IV pilus ATPase ComGA Machinery gene
  LUS72_RS21020 - 4045595..4046290 (+) 696 WP_097830695.1 helix-turn-helix transcriptional regulator -
  LUS72_RS21025 - 4046576..4046818 (+) 243 WP_000440713.1 DUF2626 domain-containing protein -
  LUS72_RS21030 - 4046927..4048321 (+) 1395 WP_097830694.1 L-cystine transporter -
  LUS72_RS21035 - 4048402..4048812 (-) 411 WP_141533531.1 hypothetical protein -
  LUS72_RS21040 - 4048883..4049098 (-) 216 WP_141533532.1 DUF3912 family protein -
  LUS72_RS21045 - 4049351..4049662 (+) 312 WP_001095549.1 hypothetical protein -
  LUS72_RS21050 - 4049697..4050185 (-) 489 WP_141533533.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39139.47 Da        Isoelectric Point: 8.6528

>NTDB_id=637891 LUS72_RS21015 WP_097830696.1 4044346..4045389(-) (comGA) [Bacillus cereus strain PT1]
MNGIELFANMIMKEACGVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGSEVYLRLSTLPTVYQESLVIRLHLQVSAQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAIRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQAIKEASGEH
VTYHYETLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=637891 LUS72_RS21015 WP_097830696.1 4044346..4045389(-) (comGA) [Bacillus cereus strain PT1]
ATGAATGGTATCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTGGGGTGCAAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGGATAGGAAAAGATTTAATTACGAAACGGTGTATTGAAAAGGAATTTGGAG
AAAAGCTTGTTTCGCACTTTAAGTTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAGCCTCAAAATGGTTCATTATAT
TTGCAAATTGATGGATCAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGTCT
CCATTTACAAGTATCTGCTCAGCCGTTGTCTCACCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCGCATGGGTTACTCGTATTTACTGGGCCGACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATCATTACACTGGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAACGAAAAAGCTGGTATCACATATAAAACGGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTATAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTCATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTTCAACTTTATGTAAATCAATGAGGC
AAGTGAGACAGGCAAGTATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATGAAACGTTGGAATCATCGGTTCGAAAAGGGTATGCTTTAGGATTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573
  pilB Glaesserella parasuis strain SC1401

43.86

82.133

0.36