Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCL44_RS10300 Genome accession   NZ_CP106809
Coordinates   1952396..1953439 (-) Length   347 a.a.
NCBI ID   WP_263071582.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain 10708     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1947396..1958439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCL44_RS10285 (OCL44_10285) - 1947775..1948059 (+) 285 WP_003692093.1 GIY-YIG nuclease family protein -
  OCL44_RS10290 (OCL44_10290) yccS 1948735..1950885 (-) 2151 WP_003697785.1 YccS family putative transporter -
  OCL44_RS10295 (OCL44_10295) - 1951002..1952232 (-) 1231 Protein_1985 PilT/PilU family type 4a pilus ATPase -
  OCL44_RS10300 (OCL44_10300) pilT 1952396..1953439 (-) 1044 WP_263071582.1 type IV pilus twitching motility protein PilT Machinery gene
  OCL44_RS10305 (OCL44_10305) - 1953559..1954245 (+) 687 WP_003690201.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCL44_RS10310 (OCL44_10310) - 1954245..1954652 (+) 408 WP_235220100.1 RNA-binding protein -
  OCL44_RS10315 (OCL44_10315) proC 1954701..1955492 (+) 792 WP_260245430.1 pyrroline-5-carboxylate reductase -
  OCL44_RS10320 (OCL44_10320) dksA 1955641..1956057 (+) 417 WP_003692108.1 RNA polymerase-binding protein DksA -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37967.34 Da        Isoelectric Point: 6.8962

>NTDB_id=637874 OCL44_RS10300 WP_263071582.1 1952396..1953439(-) (pilT) [Neisseria gonorrhoeae strain 10708]
MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSAEEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVAR
FRVNAFNTGRGPAAVFRTIPSTVLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
PIEFVHQSKKSLINQRELHQHTLSFANALNSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNGRVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQS
LQSLVRQGLIAPEAARRRAQNSESMSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=637874 OCL44_RS10300 WP_263071582.1 1952396..1953439(-) (pilT) [Neisseria gonorrhoeae strain 10708]
ATGCAGATTACCGACTTACTCGCCTTCGGCGCTAAAAACAAAGCATCCGACCTTCACCTGAGTTCGGGCATATCCCCTAT
GATTCGGGTTCACGGCGATATGCGGCGCATCAACCTTCCCGAAATGAGCGCGGAAGAGGTCGGCAATATGGTAACTTCGG
TGATGAACGACCACCAGCGGAAAATCTACCAGCAAAACTTGGAAGTCGACTTCTCGTTCGAACTGCCCAACGTCGCCCGA
TTCCGCGTCAACGCCTTCAACACCGGCCGCGGCCCCGCCGCCGTATTCCGCACCATTCCCAGCACCGTCTTATCGCTGGA
AGAATTGAAAGCCCCGAGCATTTTCCAAAAAATCGCAGAATCGCCGCGCGGCATGGTATTGGTTACCGGCCCTACCGGTT
CGGGCAAATCGACCACGCTTGCCGCGATGATCAACTACATCAACGAAACCCAGCCGGCACACATCCTGACCATCGAAGAC
CCGATCGAATTCGTCCACCAAAGCAAAAAATCCCTGATTAACCAACGCGAGCTGCACCAGCACACCCTCAGCTTCGCCAA
CGCGCTGAATTCCGCATTGCGCGAAGACCCCGACGTTATCCTTGTCGGCGAGATGCGCGACCCCGAAACCATCGGCTTGG
CACTGACCGCCGCCGAAACCGGACACTTGGTTTTCGGCACGCTGCACACGACCGGCGCGGCAAAAACCGTCGACCGTATC
GTGGACGTATTCCCCGCCGGAGAGAAAGAAATGGTGCGTTCCATGCTGTCCGAATCGCTGACCGCCGTCATCTCCCAAAA
CCTGCTGAAAACGCACGACGGCAACGGCCGTGTCGCCTCGCACGAAATCCTGATTGCCAACCCCGCCGTCCGCAACCTCA
TCCGCGAAAACAAAATCACGCAGATTAACTCCGTCCTGCAAACCGGGCAGGCGAGCGGTATGCAGACGATGGACCAATCG
CTGCAATCGCTGGTGCGCCAAGGGCTGATCGCACCGGAAGCCGCACGAAGACGCGCGCAAAACAGCGAAAGTATGAGTTT
CTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

99.712

100

0.997
  pilT Neisseria meningitidis 8013

99.135

100

0.991
  pilT Acinetobacter baumannii strain A118

67.826

99.424

0.674
  pilT Acinetobacter nosocomialis M2

67.826

99.424

0.674
  pilT Acinetobacter baumannii D1279779

67.826

99.424

0.674
  pilT Acinetobacter baylyi ADP1

67.246

99.424

0.669
  pilT Pseudomonas stutzeri DSM 10701

66.957

99.424

0.666
  pilT Pseudomonas aeruginosa PAK

66.377

99.424

0.66
  pilT Legionella pneumophila strain Lp02

64.516

98.271

0.634
  pilT Legionella pneumophila strain ERS1305867

64.516

98.271

0.634
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.242

95.101

0.611
  pilT Vibrio cholerae strain A1552

64.242

95.101

0.611
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.392

97.695

0.522
  pilU Pseudomonas stutzeri DSM 10701

40.29

99.424

0.401
  pilU Vibrio cholerae strain A1552

38.329

100

0.383
  pilU Acinetobacter baylyi ADP1

38.439

99.712

0.383