Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LUS72_RS00530 Genome accession   NZ_CP089518
Coordinates   97577..98953 (+) Length   458 a.a.
NCBI ID   WP_001085206.1    Uniprot ID   A0A2B8Z7F7
Organism   Bacillus cereus strain PT1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92577..103953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LUS72_RS00510 ctsR 92777..93238 (+) 462 WP_001244553.1 transcriptional regulator CtsR -
  LUS72_RS00515 - 93406..93954 (+) 549 WP_097833019.1 UvrB/UvrC motif-containing protein -
  LUS72_RS00520 - 93959..95023 (+) 1065 WP_097833018.1 protein arginine kinase -
  LUS72_RS00525 clpC 95046..97481 (+) 2436 WP_000971190.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  LUS72_RS00530 radA 97577..98953 (+) 1377 WP_001085206.1 DNA repair protein RadA Machinery gene
  LUS72_RS00535 disA 98957..100030 (+) 1074 WP_000392161.1 DNA integrity scanning diadenylate cyclase DisA -
  LUS72_RS00540 - 100192..101301 (+) 1110 WP_097833017.1 PIN/TRAM domain-containing protein -
  LUS72_RS00545 ispD 101318..101998 (+) 681 WP_097833016.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  LUS72_RS00550 ispF 102116..102592 (+) 477 WP_000488381.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49870.56 Da        Isoelectric Point: 7.4250

>NTDB_id=637863 LUS72_RS00530 WP_001085206.1 97577..98953(+) (radA) [Bacillus cereus strain PT1]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
SHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKPTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=637863 LUS72_RS00530 WP_001085206.1 97577..98953(+) (radA) [Bacillus cereus strain PT1]
ATGGCTAAAAAGAAAACAAAGTTTACATGTCAAGAATGTGGTTATCAGTCACCAAAATATATGGGGAAATGTCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGACGCCTTAATTATGCCAATGCAATCCAAT
CAGAAGTAACAAAACCAAGACGCCTAACAGAAGTAGAAACAAAGTCAGAGGCGCGTATTGAAACGAAATTCCAAGAGTTT
AACCGTGTACTTGGTGGTGGGATTGTAGATGGGTCCTTAGTACTTATTGGTGGAGACCCTGGGATTGGAAAATCAACATT
GCTATTACAGATTTCATCGCAATTAGCAGATTCTTCATATGATGTACTATATATATCGGGTGAGGAGTCGGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGTAATCTATTTGTTGTAGCAGAGACTGATTTACAGCGAATTGCA
TCGCACATTGAAGAGATGAATCCAGCTTTTGTTGTTATTGACTCCATTCAAACGATACATTTACCTGAAGTGACGTCAGC
ACCAGGAAGTGTCGCGCAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAGACGAAAGGGATTCCTATTTTTA
TAGTCGGACATGTGACAAAAGAAGGGGCAATTGCAGGACCACGTATGCTAGAACATATGGTTGATGCAGTTCTTTACTTT
GAAGGAGATCGTCACCATACATATCGTATTTTGCGAGCTGTGAAAAACCGTTTTGGTTCCACAAATGAAATGGGTATCTT
TGAAATGAAGGAGCTAGGTCTTGCAGAAGTACTAAACCCTTCTGAAATTTTCCTTGAAGAACGTCCAGTTGGAGTTGCAG
GATCCACAGTGGTTGCTTCAATGGAAGGAACAAGACCGGTTTTAGTAGAAATACAAGCATTAATCTCTCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACGGGAATTGATCATAACCGCGTTTCACTTATTATGGCAGTGTTAGAAAAAAGAACCGG
TTTATTGTTGCAAAATCAAGACGCGTATTTAAAAGTCGCGGGTGGTTTGAAATTAGATGAACCAGCAATCGATTTAGCCG
TTGCATTAAGTATTGCTTCAAGTTTCAGAGATAAACCTACTGCACCAACCGATGCGGTAATAGGAGAAGTAGGCCTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCGAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCAGATGGGATTGAGGTAGTGGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(60-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B8Z7F7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.946

100

0.751
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633