Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   LUY93_RS13750 Genome accession   NZ_CP089500
Coordinates   2702931..2704277 (-) Length   448 a.a.
NCBI ID   WP_000816499.1    Uniprot ID   Q9R3W9
Organism   Staphylococcus aureus strain 3144_IIV_ST8     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 2661975..2704277 2702931..2704277 within 0


Gene organization within MGE regions


Location: 2661975..2704277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LUY93_RS13515 ccrC 2661975..2663654 (+) 1680 WP_016187646.1 cassette chromosome recombinase CcrC -
  LUY93_RS13520 - 2663743..2664081 (+) 339 WP_000175892.1 SAUGI family uracil-DNA glycosylase inhibitor -
  LUY93_RS13525 - 2664175..2664486 (+) 312 WP_001166716.1 DUF960 family protein -
  LUY93_RS13530 - 2664502..2665008 (+) 507 WP_000848380.1 DUF1643 domain-containing protein -
  LUY93_RS13535 - 2665139..2665813 (+) 675 WP_001105999.1 IS6-like element IS257 family transposase -
  LUY93_RS13540 - 2665875..2668088 (+) 2214 Protein_2635 type I restriction endonuclease subunit R -
  LUY93_RS13545 - 2668168..2668734 (-) 567 WP_407545467.1 PepSY domain-containing protein -
  LUY93_RS13550 - 2668884..2669105 (-) 222 WP_001070883.1 hypothetical protein -
  LUY93_RS13555 - 2669119..2669283 (-) 165 WP_001803222.1 hypothetical protein -
  LUY93_RS13560 - 2669572..2669988 (-) 417 WP_000941331.1 hypothetical protein -
  LUY93_RS13565 - 2670050..2670577 (-) 528 WP_000717928.1 hypothetical protein -
  LUY93_RS13570 - 2670712..2671200 (-) 489 WP_000397419.1 hypothetical protein -
  LUY93_RS13575 - 2671216..2671506 (-) 291 WP_407545469.1 hypothetical protein -
  LUY93_RS13580 - 2671563..2672192 (-) 630 WP_407545470.1 transcriptional regulator -
  LUY93_RS13585 - 2672366..2673040 (-) 675 WP_001105999.1 IS6-like element IS257 family transposase -
  LUY93_RS13590 - 2673473..2673805 (-) 333 WP_001803416.1 DUF3021 domain-containing protein -
  LUY93_RS13595 - 2673834..2674076 (-) 243 WP_001803415.1 LytTR family DNA-binding domain-containing protein -
  LUY93_RS13600 - 2674208..2674882 (-) 675 WP_001106055.1 IS6-like element IS257 family transposase -
  LUY93_RS13605 tet(K) 2675079..2676458 (+) 1380 WP_000492283.1 tetracycline efflux MFS transporter Tet(K) -
  LUY93_RS13610 mobV 2676644..2677884 (+) 1241 Protein_2649 MobV family relaxase -
  LUY93_RS13615 - 2678411..2679365 (+) 955 Protein_2650 replication initiation factor domain-containing protein -
  LUY93_RS13620 - 2679454..2680128 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  LUY93_RS13625 - 2680164..2680553 (+) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  LUY93_RS13630 - 2681470..2682213 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  LUY93_RS13635 - 2682310..2682738 (+) 429 WP_000872606.1 MaoC family dehydratase -
  LUY93_RS13640 mecA 2682784..2684793 (-) 2010 WP_078056515.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  LUY93_RS13645 mecR1 2684890..2686647 (+) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  LUY93_RS13650 mecI 2686647..2687018 (+) 372 Protein_2657 mecA-type methicillin resistance repressor MecI -
  LUY93_RS13655 psm-mec 2687103..2687171 (-) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  LUY93_RS13660 - 2687491..2688639 (+) 1149 WP_000427807.1 ROK family transcriptional regulator -
  LUY93_RS13665 cstB 2688753..2690087 (-) 1335 Protein_2660 persulfide dioxygenase-sulfurtransferase CstB -
  LUY93_RS13670 - 2690120..2691184 (-) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  LUY93_RS13675 cstR 2691320..2691580 (+) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  LUY93_RS13680 - 2691580..2692218 (+) 639 Protein_2663 sulfite exporter TauE/SafE family protein -
  LUY93_RS13685 - 2692563..2693225 (-) 663 WP_001092058.1 class I SAM-dependent methyltransferase -
  LUY93_RS13690 - 2693617..2693676 (+) 60 WP_181875734.1 erythromycin resistance leader peptide -
  LUY93_RS13695 erm(A) 2693734..2694465 (+) 732 WP_001072201.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) -
  LUY93_RS13700 ant(9) 2694591..2695373 (-) 783 Protein_2667 aminoglycoside nucleotidyltransferase ANT(9) -
  LUY93_RS13705 - 2695524..2695901 (-) 378 WP_000361059.1 DUF6262 family protein -
  LUY93_RS13710 - 2695908..2697800 (-) 1893 WP_001557544.1 site-specific integrase -
  LUY93_RS13715 - 2697797..2698882 (-) 1086 WP_000868132.1 tyrosine-type recombinase/integrase -
  LUY93_RS13720 - 2699001..2699324 (-) 324 WP_030063452.1 JAB domain-containing protein -
  LUY93_RS13725 - 2699317..2699523 (-) 207 WP_001077283.1 hypothetical protein -
  LUY93_RS13730 - 2699525..2700046 (-) 522 WP_000836008.1 DUF1643 domain-containing protein -
  LUY93_RS13735 - 2700065..2700376 (-) 312 WP_000833212.1 DUF960 family protein -
  LUY93_RS13740 - 2700461..2700811 (-) 351 WP_000171449.1 SAUGI family uracil-DNA glycosylase inhibitor -
  LUY93_RS13745 ccrB 2701282..2702910 (-) 1629 WP_001186596.1 cassette chromosome recombinase CcrB Machinery gene
  LUY93_RS13750 ccrA 2702931..2704277 (-) 1347 WP_000816499.1 cassette chromosome recombinase CcrA Machinery gene

Sequence

Protein


Download         Length: 448 a.a.        Molecular weight: 52138.19 Da        Isoelectric Point: 10.1760

>NTDB_id=637672 LUY93_RS13750 WP_000816499.1 2702931..2704277(-) (ccrA) [Staphylococcus aureus strain 3144_IIV_ST8]
MKRAIGYLRQSTTKQQSLPAQKQAIELLAPKHNIQNIQYISDKQSGRTDNRTGYQQVTERIQQRQCDVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSVHDGYFDMDKAFDRLKLNIFMSLAELESDNIGEQVKNGLREKAKQGKLITTHAPFGYHYQ
NGTFIINNDESPTVKAVFNYYLQGYGYKKIAQYLEDDNKLITRKPYQVRNIIMNPNYCGRVINQYGQYNNMVPPIVSATK
YEHAQAIRNKKQLHCIPSENQLKQKIKCPCCDSTLTNMTIRKKHTLRYYICPKNMNESRFVCSFKGINAQKLEVQVLATC
QNFFQNQQLYSKINNAIHQRLKKQRVIEAKSTLTQEQLIDKLAKGMIDAESFRKQTHLMNQKHKTISSISDNQLQTSLQK
VIQKSFTLNMLHPYIDEIRITKNKALVGIYFKNEPLNIVNQTSQSSIA

Nucleotide


Download         Length: 1347 bp        

>NTDB_id=637672 LUY93_RS13750 WP_000816499.1 2702931..2704277(-) (ccrA) [Staphylococcus aureus strain 3144_IIV_ST8]
ATGAAACGAGCCATTGGTTATTTGCGCCAAAGTACAACGAAACAACAATCACTCCCAGCTCAAAAGCAAGCAATAGAATT
ATTAGCTCCAAAGCACAATATTCAAAATATCCAATACATTAGTGATAAGCAATCAGGCAGAACAGATAATCGAACAGGCT
ATCAACAAGTCACCGAACGCATCCAACAAAGACAATGTGACGTATTATGTTGTTATCGCTTGAATCGACTTCATCGCAAC
TTGAAAAATGCATTAAAACTCATGAAACTCTGTCAAAAATATCATGTTCATATTCTAAGTGTTCATGATGGCTATTTTGA
TATGGATAAAGCGTTTGATCGCCTAAAACTCAATATATTCATGAGTCTGGCTGAACTTGAATCCGATAATATTGGAGAAC
AAGTCAAAAATGGACTTAGAGAAAAGGCAAAACAAGGTAAACTCATAACGACCCATGCGCCTTTCGGTTATCACTATCAA
AATGGTACTTTCATCATTAATAATGATGAATCACCTACCGTCAAAGCTGTATTCAATTATTATCTTCAAGGATATGGCTA
CAAGAAGATTGCACAATATTTAGAAGACGATAATAAACTTATTACCCGCAAGCCTTATCAGGTACGAAATATAATTATGA
ACCCAAATTATTGTGGTCGTGTCATCAATCAATATGGTCAATATAACAATATGGTACCACCTATTGTTTCGGCAACGAAA
TATGAACATGCTCAAGCAATCCGTAATAAGAAGCAACTTCACTGTATACCTTCAGAGAATCAGCTGAAACAAAAGATCAA
ATGTCCTTGTTGTGACTCAACACTGACAAATATGACAATAAGAAAAAAACATACATTGCGATATTATATTTGTCCTAAAA
ATATGAATGAATCTCGCTTTGTCTGTTCATTCAAAGGAATAAATGCACAAAAATTAGAAGTTCAAGTCTTAGCTACATGT
CAGAACTTCTTTCAAAACCAACAGCTCTATTCAAAAATTAATAATGCAATTCATCAACGCCTCAAAAAACAAAGAGTGAT
AGAAGCTAAAAGTACGCTAACTCAAGAACAACTGATAGATAAACTTGCCAAAGGTATGATTGATGCTGAATCATTCAGAA
AACAGACTCATTTGATGAATCAAAAGCACAAAACCATATCCTCCATAAGTGATAATCAGTTACAAACATCACTACAAAAG
GTTATACAGAAAAGTTTCACGTTAAACATGCTGCATCCCTATATTGATGAAATTCGCATTACAAAAAATAAAGCCCTTGT
TGGGATCTATTTCAAAAATGAACCATTGAACATTGTGAACCAAACCTCGCAATCATCGATTGCTTAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(168-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9R3W9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

78.174

100

0.783
  ccrA Staphylococcus aureus N315

72.383

100

0.725