Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   LUX28_RS13045 Genome accession   NZ_CP089310
Coordinates   2638180..2639217 (-) Length   345 a.a.
NCBI ID   WP_053573197.1    Uniprot ID   -
Organism   Bacillus velezensis strain A4P130     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2633180..2644217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LUX28_RS12995 (LUX28_12995) tasA 2633414..2634199 (-) 786 WP_007408329.1 biofilm matrix protein TasA -
  LUX28_RS13000 (LUX28_13000) sipW 2634264..2634848 (-) 585 WP_015240205.1 signal peptidase I SipW -
  LUX28_RS13005 (LUX28_13005) tapA 2634820..2635491 (-) 672 WP_053573199.1 amyloid fiber anchoring/assembly protein TapA -
  LUX28_RS13010 (LUX28_13010) - 2635750..2636079 (+) 330 WP_039254490.1 DUF3889 domain-containing protein -
  LUX28_RS13015 (LUX28_13015) - 2636119..2636298 (-) 180 WP_003153093.1 YqzE family protein -
  LUX28_RS13020 (LUX28_13020) comGG 2636355..2636732 (-) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  LUX28_RS13025 (LUX28_13025) comGF 2636733..2637233 (-) 501 WP_256052909.1 competence type IV pilus minor pilin ComGF -
  LUX28_RS13030 (LUX28_13030) comGE 2637142..2637456 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  LUX28_RS13035 (LUX28_13035) comGD 2637440..2637877 (-) 438 WP_012117983.1 competence type IV pilus minor pilin ComGD Machinery gene
  LUX28_RS13040 (LUX28_13040) comGC 2637867..2638175 (-) 309 WP_079979070.1 competence type IV pilus major pilin ComGC Machinery gene
  LUX28_RS13045 (LUX28_13045) comGB 2638180..2639217 (-) 1038 WP_053573197.1 competence type IV pilus assembly protein ComGB Machinery gene
  LUX28_RS13050 (LUX28_13050) comGA 2639204..2640274 (-) 1071 WP_053573196.1 competence type IV pilus ATPase ComGA Machinery gene
  LUX28_RS13055 (LUX28_13055) - 2640467..2641417 (-) 951 WP_015417820.1 magnesium transporter CorA family protein -
  LUX28_RS13060 (LUX28_13060) - 2641563..2642864 (+) 1302 WP_012117986.1 hemolysin family protein -
  LUX28_RS13065 (LUX28_13065) - 2642902..2643732 (-) 831 WP_053573195.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39586.97 Da        Isoelectric Point: 9.6788

>NTDB_id=636407 LUX28_RS13045 WP_053573197.1 2638180..2639217(-) (comGB) [Bacillus velezensis strain A4P130]
MKRIKRTWPLKDQAAFLKRLGGMTEKGYSLIEGLRLLQLQLHKRQLAELKEGIRRLREGDAFYQVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQIKKMLRYPMFLIFSVCVMFYILQSLIIPQFSGIYQSMNMNTSGATAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPCYEKDLAAAVSHGQANGLLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=636407 LUX28_RS13045 WP_053573197.1 2638180..2639217(-) (comGB) [Bacillus velezensis strain A4P130]
ATGAAGCGGATTAAACGAACATGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGGAATGACGGAAAAAGG
ATACAGCCTGATTGAAGGGCTCAGACTGCTGCAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGAAGGAAGGAATCC
GCCGGCTGAGAGAAGGTGATGCGTTTTACCAAGTGCTTGAGGCACTGTCTTTTCATAAAGAAGCCGTCTCTATCTGCTAT
TTCGCGGAGAAACACGGGGAATTGCCGGGGGCGATGAAACAAAGCGGCGAATTATTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTTTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACGTCCGGTGCGACTGCTTTCATCTTTGCATTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTTTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTGATTGTTGTGAAAATACCACTATTAGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCGATCTATGACAGCTTAACAGCTTTTAAAGAGCAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTGTTATGAAAAAGACCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCTGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.635

93.623

0.614