Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LUX28_RS09025 Genome accession   NZ_CP089310
Coordinates   1845893..1846327 (+) Length   144 a.a.
NCBI ID   WP_053573457.1    Uniprot ID   -
Organism   Bacillus velezensis strain A4P130     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1840893..1851327
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LUX28_RS09020 (LUX28_09020) - 1844393..1845604 (-) 1212 WP_039254928.1 cytochrome P450 family protein -
  LUX28_RS09025 (LUX28_09025) nucA/comI 1845893..1846327 (+) 435 WP_053573457.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  LUX28_RS09030 (LUX28_09030) - 1846387..1847142 (+) 756 WP_003154084.1 YoaK family protein -
  LUX28_RS09035 (LUX28_09035) - 1847171..1847533 (-) 363 WP_053573458.1 hypothetical protein -
  LUX28_RS09040 (LUX28_09040) - 1847726..1849054 (-) 1329 WP_015417518.1 S8 family peptidase -
  LUX28_RS09045 (LUX28_09045) - 1849233..1849466 (+) 234 WP_015239897.1 hypothetical protein -
  LUX28_RS09050 (LUX28_09050) - 1849722..1850429 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  LUX28_RS09055 (LUX28_09055) - 1850489..1850941 (+) 453 WP_014305039.1 OsmC family protein -
  LUX28_RS09060 (LUX28_09060) - 1850955..1851308 (-) 354 WP_007410379.1 DMT family transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15548.46 Da        Isoelectric Point: 7.2418

>NTDB_id=636398 LUX28_RS09025 WP_053573457.1 1845893..1846327(+) (nucA/comI) [Bacillus velezensis strain A4P130]
MNAFMKWAASLLLVISLQFGFTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=636398 LUX28_RS09025 WP_053573457.1 1845893..1846327(+) (nucA/comI) [Bacillus velezensis strain A4P130]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCTTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCAGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

59.829

81.25

0.486