Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   LT232_RS00620 Genome accession   NZ_CP089287
Coordinates   100951..101718 (+) Length   255 a.a.
NCBI ID   WP_015240232.1    Uniprot ID   -
Organism   Bacillus velezensis strain GSBZ09     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 95951..106718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LT232_RS00590 (LT232_00590) - 96235..98367 (+) 2133 WP_094031843.1 HD family phosphohydrolase -
  LT232_RS00595 (LT232_00595) ybeY 98368..98841 (+) 474 WP_012118016.1 rRNA maturation RNase YbeY -
  LT232_RS00600 (LT232_00600) - 98822..99196 (+) 375 WP_015240233.1 diacylglycerol kinase family protein -
  LT232_RS00605 (LT232_00605) - 99320..99730 (+) 411 WP_003152976.1 cytidine deaminase -
  LT232_RS00610 (LT232_00610) era 99711..100616 (+) 906 WP_094031842.1 GTPase Era -
  LT232_RS00615 (LT232_00615) - 100771..100914 (+) 144 WP_007408286.1 YqzL family protein -
  LT232_RS00620 (LT232_00620) recO 100951..101718 (+) 768 WP_015240232.1 DNA repair protein RecO Machinery gene
  LT232_RS00625 (LT232_00625) glyQ 102020..102907 (+) 888 WP_094031841.1 glycine--tRNA ligase subunit alpha -
  LT232_RS00630 (LT232_00630) glyS 102900..104939 (+) 2040 WP_059368104.1 glycine--tRNA ligase subunit beta -
  LT232_RS00635 (LT232_00635) ccpN 105040..105678 (+) 639 WP_003152989.1 transcriptional regulator CcpN -
  LT232_RS00640 (LT232_00640) - 105704..106516 (+) 813 WP_003152991.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29294.89 Da        Isoelectric Point: 8.7092

>NTDB_id=636128 LT232_RS00620 WP_015240232.1 100951..101718(+) (recO) [Bacillus velezensis strain GSBZ09]
MLTKSEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPMSPQTARLLRLFYYFDIGRLGSVSLKQETKNEIKRVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=636128 LT232_RS00620 WP_015240232.1 100951..101718(+) (recO) [Bacillus velezensis strain GSBZ09]
ATGCTGACTAAAAGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGACGTGATCACCTTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGGTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATGTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CCAAAAACGAAATTAAACGGGTCATTGATCTGTATTACGAGGAATATTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GATCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

88.235

100

0.882