Detailed information NTG0635983

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LSG45_RS02155
• Genome accession: NZ_CP089238
• Coordinates: 451858..453006 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain JNQH-PA033
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 446858..458006

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LSG45_RS02130 (LSG45_02130)	-	448009..448305 (-)	297	WP_234575792.1	DUF167 domain-containing protein	-
LSG45_RS02135 (LSG45_02135)	-	448302..448895 (-)	594	WP_003084544.1	YggT family protein	-
LSG45_RS02140 (LSG45_02140)	proC	448906..449727 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
LSG45_RS02145 (LSG45_02145)	-	449739..450431 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
LSG45_RS02150 (LSG45_02150)	pilT	450646..451680 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
LSG45_RS02155 (LSG45_02155)	pilU	451858..453006 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
LSG45_RS02160 (LSG45_02160)	czcD	453014..453913 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
LSG45_RS02165 (LSG45_02165)	-	454067..454471 (+)	405	WP_003084560.1	NINE protein	-
LSG45_RS02170 (LSG45_02170)	-	454714..456087 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
LSG45_RS02175 (LSG45_02175)	-	456084..457268 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=635983 LSG45_RS02155 WP_003110016.1 451858..453006(+) (pilU) [Pseudomonas aeruginosa strain JNQH-PA033]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=635983 LSG45_RS02155 WP_003110016.1 451858..453006(+) (pilU) [Pseudomonas aeruginosa strain JNQH-PA033]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382