Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   N7985_RS12835 Genome accession   NZ_CP106673
Coordinates   2500072..2500482 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain SRCM116268     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2495072..2505482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7985_RS12805 (N7985_12805) - 2495159..2495299 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  N7985_RS12810 (N7985_12810) yqeF 2495604..2496335 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  N7985_RS12815 (N7985_12815) cwlH 2496587..2497339 (-) 753 WP_021480067.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  N7985_RS12820 (N7985_12820) yqeD 2497526..2498152 (+) 627 WP_041850032.1 TVP38/TMEM64 family protein -
  N7985_RS12825 (N7985_12825) gnd 2498172..2499065 (-) 894 WP_041850033.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  N7985_RS12830 (N7985_12830) yqeB 2499317..2500039 (+) 723 WP_032726205.1 hypothetical protein -
  N7985_RS12835 (N7985_12835) nucA/comI 2500072..2500482 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  N7985_RS12840 (N7985_12840) - 2500678..2501028 (+) 351 Protein_2486 sigma-70 family RNA polymerase sigma factor -
  N7985_RS12845 (N7985_12845) spoIVCA 2501056..2502516 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  N7985_RS12850 (N7985_12850) - 2502474..2502652 (-) 179 Protein_2488 hypothetical protein -
  N7985_RS12855 (N7985_12855) arsC 2503007..2503426 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  N7985_RS12860 (N7985_12860) acr3 2503438..2504478 (-) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  N7985_RS12865 (N7985_12865) arsK 2504501..2504941 (-) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  N7985_RS12870 (N7985_12870) arsR 2505000..2505317 (-) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=635524 N7985_RS12835 WP_009967785.1 2500072..2500482(-) (nucA/comI) [Bacillus subtilis strain SRCM116268]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=635524 N7985_RS12835 WP_009967785.1 2500072..2500482(-) (nucA/comI) [Bacillus subtilis strain SRCM116268]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529