Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   N7980_RS08275 Genome accession   NZ_CP106671
Coordinates   1592871..1593281 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain SRCM116301     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1587871..1598281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7980_RS08240 (N7980_08240) arsR 1588036..1588353 (+) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -
  N7980_RS08245 (N7980_08245) arsK 1588412..1588852 (+) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  N7980_RS08250 (N7980_08250) acr3 1588875..1589915 (+) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  N7980_RS08255 (N7980_08255) arsC 1589927..1590346 (+) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  N7980_RS08260 (N7980_08260) - 1590701..1590879 (+) 179 Protein_1618 hypothetical protein -
  N7980_RS08265 (N7980_08265) spoIVCA 1590837..1592297 (+) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  N7980_RS08270 (N7980_08270) - 1592325..1592675 (-) 351 Protein_1620 sigma-70 family RNA polymerase sigma factor -
  N7980_RS08275 (N7980_08275) nucA/comI 1592871..1593281 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  N7980_RS08280 (N7980_08280) yqeB 1593314..1594036 (-) 723 WP_032726205.1 hypothetical protein -
  N7980_RS08285 (N7980_08285) gnd 1594288..1595181 (+) 894 WP_041850033.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  N7980_RS08290 (N7980_08290) yqeD 1595201..1595827 (-) 627 WP_041850032.1 TVP38/TMEM64 family protein -
  N7980_RS08295 (N7980_08295) cwlH 1596014..1596766 (+) 753 WP_021480067.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  N7980_RS08300 (N7980_08300) yqeF 1597018..1597749 (+) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  N7980_RS08305 (N7980_08305) - 1598054..1598194 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=635322 N7980_RS08275 WP_009967785.1 1592871..1593281(+) (nucA/comI) [Bacillus subtilis strain SRCM116301]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=635322 N7980_RS08275 WP_009967785.1 1592871..1593281(+) (nucA/comI) [Bacillus subtilis strain SRCM116301]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529