Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LRO88_RS14315 Genome accession   NZ_CP089148
Coordinates   2615540..2615950 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain NIB353     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2610540..2620950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRO88_RS14285 - 2610626..2610766 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  LRO88_RS14290 yqeF 2611072..2611803 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  LRO88_RS14295 cwlH 2612055..2612807 (-) 753 WP_021480067.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  LRO88_RS14300 yqeD 2612994..2613620 (+) 627 WP_159376965.1 TVP38/TMEM64 family protein -
  LRO88_RS14305 gnd 2613640..2614533 (-) 894 WP_046160611.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  LRO88_RS14310 yqeB 2614785..2615507 (+) 723 WP_014480321.1 hypothetical protein -
  LRO88_RS14315 nucA/comI 2615540..2615950 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  LRO88_RS14320 sigK 2616146..2616874 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  LRO88_RS14325 - 2616874..2616990 (+) 117 WP_041850087.1 hypothetical protein -
  LRO88_RS14330 - 2616956..2617153 (-) 198 Protein_2783 recombinase family protein -
  LRO88_RS14335 - 2617111..2617289 (-) 179 Protein_2784 hypothetical protein -
  LRO88_RS14340 psiE 2617830..2618246 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  LRO88_RS14345 yrkQ 2618292..2619590 (-) 1299 WP_049141120.1 two-component system sensor histidine kinase YrkQ -
  LRO88_RS14350 yrkP 2619577..2620272 (-) 696 WP_021480140.1 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=635317 LRO88_RS14315 WP_009967785.1 2615540..2615950(-) (nucA/comI) [Bacillus subtilis strain NIB353]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=635317 LRO88_RS14315 WP_009967785.1 2615540..2615950(-) (nucA/comI) [Bacillus subtilis strain NIB353]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529