Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   LRO88_RS00020 Genome accession   NZ_CP089148
Coordinates   3028..4140 (+) Length   370 a.a.
NCBI ID   WP_159376581.1    Uniprot ID   -
Organism   Bacillus subtilis strain NIB353     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRO88_RS00005 dnaA 1..1341 (+) 1341 WP_003242674.1 chromosomal replication initiator protein DnaA -
  LRO88_RS00010 dnaN 1530..2666 (+) 1137 WP_003242509.1 DNA polymerase III subunit beta -
  LRO88_RS00015 rlbA 2797..3012 (+) 216 WP_003226810.1 ribosome maturation protein RlbA -
  LRO88_RS00020 recF 3028..4140 (+) 1113 WP_159376581.1 DNA replication/repair protein RecF Machinery gene
  LRO88_RS00025 remB 4158..4403 (+) 246 WP_003219266.1 extracellular matrix regulator RemB -
  LRO88_RS00030 gyrB 4458..6374 (+) 1917 WP_003226808.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  LRO88_RS00035 gyrA 6585..9050 (+) 2466 WP_003244540.1 DNA topoisomerase (ATP-hydrolyzing) subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42289.37 Da        Isoelectric Point: 7.3660

>NTDB_id=635264 LRO88_RS00020 WP_159376581.1 3028..4140(+) (recF) [Bacillus subtilis strain NIB353]
MYILNLELTSYRNYDHAELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDKDYAKIEGRVMKQNGA
IPMQLVISKKGKKGKVNHIEQQKLSQYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLHDLSLYQKILSQR
NHFLKQLQTRKQTDRTMLDVLTDQLVEVAAKVVVKRLQFTAQLEKWAQPIHAGISRGLEELTLKYHTALDVSDPLDLSKI
GDSYQEAFSKLREKEIERGVTLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHETLRQAGMFRVQNGALVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=635264 LRO88_RS00020 WP_159376581.1 3028..4140(+) (recF) [Bacillus subtilis strain NIB353]
TTGTATATCCTGAACTTAGAACTGACATCTTACCGCAACTACGACCATGCTGAACTTCAATTTGAAAATAAAGTAAATGT
GATCATCGGAGAAAACGCCCAGGGGAAGACAAACCTCATGGAGGCGATCTATGTCTTGTCCATGGCGAAATCGCACCGGA
CATCAAATGACAAAGAACTTATACGATGGGACAAAGACTATGCTAAAATAGAGGGAAGAGTGATGAAGCAAAACGGGGCG
ATCCCGATGCAGCTCGTCATCTCCAAAAAGGGTAAAAAGGGCAAGGTCAATCATATTGAACAGCAAAAGCTCAGCCAGTA
TGTCGGGGCCCTCAACACGATTATGTTCGCGCCGGAAGATTTAAATCTTGTAAAAGGAAGCCCTCAAGTAAGAAGGCGGT
TTCTTGACATGGAAATCGGACAGGTTTCTCCCGTCTACCTTCATGATCTTTCTCTTTACCAGAAAATCCTTTCCCAGCGG
AATCATTTTTTGAAACAGCTGCAAACAAGAAAACAAACTGACCGGACGATGCTCGATGTTCTGACCGATCAGCTTGTAGA
AGTTGCAGCAAAAGTCGTCGTAAAACGCCTGCAGTTTACAGCACAGCTCGAGAAATGGGCGCAGCCCATCCATGCAGGCA
TCTCAAGAGGGCTTGAAGAACTGACCCTGAAATACCATACAGCTCTTGATGTATCAGATCCCCTAGATTTGTCGAAAATA
GGAGATAGCTATCAAGAAGCGTTTTCTAAATTAAGAGAAAAAGAAATTGAGCGTGGTGTGACGCTGTCAGGGCCTCATCG
CGATGATGTTCTTTTCTATGTGAACGGACGCGATGTGCAGACGTATGGTTCTCAAGGACAGCAGCGAACGACGGCGTTGT
CCCTTAAGCTGGCGGAGATTGACCTGATCCATGAAGAAATCGGAGAATATCCCATTTTACTATTGGATGATGTACTGAGT
GAACTGGATGATTATCGCCAGTCACACTTGCTTCATACGATCCAAGGCCGTGTACAAACGTTTGTCACAACGACAAGCGT
TGATGGCATTGATCACGAAACCTTACGTCAAGCAGGAATGTTCCGTGTGCAAAATGGTGCGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-350)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

99.73

100

0.997