Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N2X48_RS23155 Genome accession   NZ_CP104913
Coordinates   4992870..4994363 (+) Length   497 a.a.
NCBI ID   WP_003094357.1    Uniprot ID   A0A0H2ZGR9
Organism   Pseudomonas aeruginosa strain PA-AUTBAM     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4987870..4999363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N2X48_RS23125 (N2X48_23125) - 4988082..4988891 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  N2X48_RS23130 (N2X48_23130) kdsD 4989305..4990285 (+) 981 WP_003134758.1 arabinose-5-phosphate isomerase KdsD -
  N2X48_RS23135 (N2X48_23135) - 4990285..4990824 (+) 540 WP_003112742.1 KdsC family phosphatase -
  N2X48_RS23140 (N2X48_23140) lptC 4990833..4991405 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  N2X48_RS23145 (N2X48_23145) lptA 4991392..4991919 (+) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  N2X48_RS23150 (N2X48_23150) lptB 4991919..4992644 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  N2X48_RS23155 (N2X48_23155) rpoN/rpoN1 4992870..4994363 (+) 1494 WP_003094357.1 RNA polymerase factor sigma-54 Machinery gene
  N2X48_RS23160 (N2X48_23160) hpf 4994441..4994749 (+) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  N2X48_RS23165 (N2X48_23165) ptsN 4994763..4995227 (+) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  N2X48_RS23170 (N2X48_23170) rapZ 4995229..4996089 (+) 861 WP_003094361.1 RNase adapter RapZ -
  N2X48_RS23175 (N2X48_23175) - 4996119..4996391 (+) 273 WP_003094362.1 HPr family phosphocarrier protein -
  N2X48_RS23180 (N2X48_23180) - 4996488..4997420 (-) 933 WP_003120918.1 ZIP family metal transporter -
  N2X48_RS23185 (N2X48_23185) - 4997439..4998050 (-) 612 WP_003098862.1 superoxide dismutase -
  N2X48_RS23190 (N2X48_23190) - 4998063..4998512 (-) 450 WP_003094369.1 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56054.22 Da        Isoelectric Point: 4.4327

>NTDB_id=635060 N2X48_RS23155 WP_003094357.1 4992870..4994363(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain PA-AUTBAM]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=635060 N2X48_RS23155 WP_003094357.1 4992870..4994363(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain PA-AUTBAM]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCTCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCCCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAGTGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTACCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCGCGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGATAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCATGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCTGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZGR9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477